| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,280,527 | A→G | 24.2% | S332G (AGC→GGC) | narK → | nitrate/nitrite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,280,527 | 0 | A | G | 24.2% | 57.6 / 15.8 | 33 | S332G (AGC→GGC) | narK | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base A (11/14); new base G (5/3); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCT > W3110S.gb/1280461‑1280596 | gCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCGGCg > 1:1269011/1‑70 (MQ=255) cATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGc > 1:2688909/1‑71 (MQ=255) aTCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCGGCGGCctgc > 1:1482278/1‑71 (MQ=255) aTCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGGctg > 1:271992/1‑70 (MQ=255) tGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCctgct > 1:553454/1‑69 (MQ=255) tGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCctgct > 1:1837076/1‑69 (MQ=255) tGACCGTCTGGGCGAAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCctgct > 1:2285455/1‑69 (MQ=255) ccGTCTGGGCGGAACTCGTGTCACGCTGGTGAATTTTATTCTGATGGCGATTTTCGGCGGCCTGCTGTTcc > 1:1591526/1‑71 (MQ=255) ggCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCt > 1:1561320/1‑70 (MQ=255) tCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTc > 1:1534920/1‑55 (MQ=255) tCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTc > 1:547466/1‑55 (MQ=255) gtCACGCTGGTGAACTTTATTCTGATGGCGATTTTAGGCGGCCTGCTGTTCCt > 1:335469/1‑53 (MQ=37) tCACGCTGGTGAACTTTATTCTGATGGCGATTTTCGGCGGCCTGCTGTTCCTGACCTTACCGACTGACggg < 1:1032719/71‑1 (MQ=255) aCGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACggg > 1:3993880/1‑69 (MQ=255) gTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTcc < 1:1714911/43‑1 (MQ=255) gTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTcc < 1:2819079/43‑1 (MQ=255) gTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCt < 1:2078911/49‑1 (MQ=255) gTGAACTTTATTCTGATGGCGACTTTCAGCGGCCTGCTGTTcc < 1:3318687/43‑1 (MQ=255) tGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACggg < 1:3683086/62‑1 (MQ=255) cTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACt < 1:1128766/52‑1 (MQ=255) tATTCTGATGGCGATTTTCGGCGGCCTGCTGTTGCTGACCTTACCGACTGACGGGCAGGGCGGAAGCtt < 1:1627149/69‑1 (MQ=255) tATTCTGATGGCGATTTTCGGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCtt < 1:42540/69‑1 (MQ=255) tATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCtt < 1:2046886/69‑1 (MQ=255) tATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCa > 1:2878330/1‑71 (MQ=255) ttCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAg < 1:1467680/64‑1 (MQ=255) ttCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAg < 1:3394000/64‑1 (MQ=255) gATGGCGATTTTCGGCGGCCTGCTGTTCCTGACCTTAc > 1:3973757/1‑38 (MQ=255) aTTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGc < 1:892610/43‑1 (MQ=255) aTTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGc < 1:263317/43‑1 (MQ=255) aTTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGc < 1:2611507/43‑1 (MQ=255) aTTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGc < 1:1506428/43‑1 (MQ=255) aTTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGc < 1:1440277/43‑1 (MQ=255) attCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCa > 1:25227/2‑42 (MQ=255) aGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCt < 1:3582709/70‑1 (MQ=255) aGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCt < 1:1527928/70‑1 (MQ=255) | GCATTATCTGACCGTCTGGGCGGAACTCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCT > W3110S.gb/1280461‑1280596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |