| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,282,409 | A→G | 20.0% | P323P (CCA→CCG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,282,409 | 0 | A | G | 20.0% | 59.5 / 11.5 | 30 | P323P (CCA→CCG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (13/11); new base G (2/4); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
CACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGTGATGCTGG > W3110S.gb/1282343‑1282470 | cACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGc > 1:557863/1‑70 (MQ=255) aTGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAt < 1:1856813/71‑1 (MQ=255) cGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAttt < 1:207650/70‑1 (MQ=255) cGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTAttt < 1:2778171/70‑1 (MQ=255) ggCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGAc > 1:45321/1‑70 (MQ=255) ggCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCAAAGCCAGTATTTCACCGa > 1:634167/1‑69 (MQ=255) gcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAtg < 1:726967/71‑1 (MQ=255) cTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAtg < 1:558670/70‑1 (MQ=255) atGGCGATGGGCCACGTAATGCTGCGTGAAATCCACCTCGACAACCCAAGCCAGTATTTCACCGACTAtg < 1:2474489/69‑1 (MQ=255) tGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGc < 1:3697611/71‑1 (MQ=255) tGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGc < 1:3702175/71‑1 (MQ=255) tGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGc < 1:2185175/71‑1 (MQ=255) tGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGc < 1:302975/71‑1 (MQ=255) gTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCa > 1:596079/1‑38 (MQ=255) aaTGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACa > 1:877148/1‑71 (MQ=255) atgatgCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGAc < 1:3967294/69‑1 (MQ=255) aTGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACa < 1:442204/70‑1 (MQ=255) tgctgcGTGAATTCCACCTCGACAACCCAAGCCAg < 1:295104/35‑1 (MQ=255) ctgcGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGcc > 1:2543076/1‑71 (MQ=255) tgcGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGcc > 1:2502845/1‑70 (MQ=255) tGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACt < 1:3981765/41‑1 (MQ=255) tGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACt < 1:2219490/41‑1 (MQ=255) ccACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGa > 1:2939828/1‑62 (MQ=255) ccACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGa > 1:278995/1‑62 (MQ=255) cACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGTGAt > 1:1080148/1‑71 (MQ=255) cACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGTGAt > 1:2481910/1‑71 (MQ=255) gACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa > 1:3871870/1‑47 (MQ=255) gACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa > 1:1130219/1‑47 (MQ=255) gACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGTGATGCTgg > 1:2187306/1‑70 (MQ=255) aaCCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGa > 1:2238845/1‑52 (MQ=255) | CACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCTGGTGATGCTGG > W3110S.gb/1282343‑1282470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |