Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,303,631 C→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,303,6310CT100.0% 145.7 / NA 50Q246Y (CAG→TAT) oppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/30);  total (20/30)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATC  >  W3110S.gb/1303566‑1303696
                                                                 |                                                                 
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                              <  1:3441375/71‑1 (MQ=255)
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                              <  1:1918543/71‑1 (MQ=255)
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                              <  1:1913936/71‑1 (MQ=255)
     gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                         <  1:249080/71‑1 (MQ=255)
     gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                         <  1:2146871/71‑1 (MQ=255)
     gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                         <  1:3111321/71‑1 (MQ=255)
         gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                              <  1:860883/62‑1 (MQ=255)
         gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTg                                                     >  1:2208225/1‑71 (MQ=255)
         gaaTCGTTCATGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTg                                                     >  1:3730641/1‑71 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                   <  1:2907703/71‑1 (MQ=255)
           aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                   <  1:1752764/71‑1 (MQ=255)
              gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                                >  1:675062/1‑71 (MQ=255)
                 cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                       >  1:3041864/1‑61 (MQ=255)
                 cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                       >  1:1214677/1‑61 (MQ=255)
                      aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                        <  1:2644929/71‑1 (MQ=255)
                              cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:732601/71‑1 (MQ=255)
                              cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:2331447/71‑1 (MQ=255)
                              cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:1169042/71‑1 (MQ=255)
                              cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGAAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:1247352/71‑1 (MQ=255)
                                aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                              <  1:947748/71‑1 (MQ=255)
                                    aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                             >  1:2055262/1‑68 (MQ=255)
                                    aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                             >  1:3982245/1‑68 (MQ=255)
                                    aCTGGAACAACGCGAAAACCGTTATTAACTATGTAAACTATTTGCCTATTGCTTCTGAAGTTACCGATGt                           >  1:884637/1‑70 (MQ=255)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                       >  1:2139246/1‑41 (MQ=38)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                          >  1:3227605/1‑70 (MQ=255)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                          >  1:3619537/1‑70 (MQ=255)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                          <  1:3236235/70‑1 (MQ=255)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                          >  1:1298878/1‑70 (MQ=255)
                                      tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                              <  1:434899/65‑1 (MQ=255)
                                       ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                       >  1:1185987/1‑71 (MQ=255)
                                        gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg                            >  1:1235680/1‑65 (MQ=255)
                                             acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:3920445/56‑1 (MQ=255)
                                             acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:3804533/56‑1 (MQ=255)
                                             acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:3880788/56‑1 (MQ=255)
                                             acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAc                 >  1:3180559/1‑71 (MQ=255)
                                                cgAAAACCGTTATTAACTATGTCACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt              <  1:611686/71‑1 (MQ=255)
                                                cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt              <  1:4056358/71‑1 (MQ=255)
                                                cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt              <  1:1348854/71‑1 (MQ=255)
                                                 gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:1093843/52‑1 (MQ=255)
                                                 gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:3671376/52‑1 (MQ=255)
                                                 gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:1715165/52‑1 (MQ=255)
                                                    aaCCGTTATTAACTATTTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                              <  1:3698828/51‑1 (MQ=255)
                                                       cGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggt        >  1:2334292/1‑70 (MQ=255)
                                                         ttattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt              <  1:3016351/62‑1 (MQ=255)
                                                           attaACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                     >  1:1450489/1‑37 (MQ=37)
                                                           attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                                  >  1:3081539/1‑40 (MQ=38)
                                                           attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt   >  1:1727962/1‑71 (MQ=255)
                                                           attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt   >  1:893096/1‑71 (MQ=255)
                                                            ttaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCg                               <  1:3237599/42‑1 (MQ=38)
                                                            ttaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATc  <  1:3121996/71‑1 (MQ=255)
                                                                 |                                                                 
TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATC  >  W3110S.gb/1303566‑1303696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: