| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,309,136 | C→T | 12.7% | V408V (GTG→GTA) | cls ← | cardiolipin synthase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,309,136 | 0 | C | T | 12.7% | 175.9 / 14.4 | 63 | V408V (GTG→GTA) | cls | cardiolipin synthase 1 |
| Reads supporting (aligned to +/- strand): ref base C (32/23); new base T (2/6); total (34/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGT > W3110S.gb/1309067‑1309204 | gAAATTTAGCCACAGACTACGGATATCAAGGTTAACTGTGACAACCAGACTTAGTTCGCCATCGACCAGCa > 1:1202677/1‑71 (MQ=255)gAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCa > 1:322396/1‑71 (MQ=255)gAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCa > 1:2692307/1‑71 (MQ=255)gAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCa > 1:1521835/1‑71 (MQ=255) tAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGTACGctct > 1:1722741/1‑71 (MQ=255) tAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGctct > 1:3551692/1‑71 (MQ=255) tAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGACATCGACCAGTACGctct > 1:3835975/1‑71 (MQ=255) gCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGTAAGCTCTTg < 1:1438839/71‑1 (MQ=255) ccACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGTACGCTCTTgg < 1:1398204/71‑1 (MQ=255) ccACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGTACGCTCTTgg < 1:369205/71‑1 (MQ=255) ccACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTgg < 1:2432240/71‑1 (MQ=255) ccACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTgg < 1:330144/71‑1 (MQ=255) ccACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTgg < 1:3567508/71‑1 (MQ=255) ccACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTgg < 1:292068/71‑1 (MQ=255) cacaGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGTACGCTCTTgg < 1:410223/70‑1 (MQ=255) cacaGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTgg < 1:1806656/70‑1 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATg < 1:2026772/59‑1 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATg < 1:2845377/59‑1 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATg < 1:2293501/59‑1 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATg < 1:71733/59‑1 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATg < 1:3717719/59‑1 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATg < 1:1536918/59‑1 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATg < 1:4028451/59‑1 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTaa > 1:3674074/1‑65 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTaa > 1:453907/1‑65 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTaa > 1:1050585/1‑65 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTaa > 1:1582382/1‑65 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTaa > 1:1627224/1‑65 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTaa > 1:1891989/1‑65 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGcc > 1:630558/1‑71 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGcc > 1:45775/1‑71 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGcc > 1:3717512/1‑71 (MQ=255) tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGcc > 1:3100141/1‑71 (MQ=255) aaGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGc > 1:2247264/1‑47 (MQ=255) aaGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGc > 1:2921463/1‑47 (MQ=255) aaGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGc > 1:112092/1‑58 (MQ=255) aaGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCtt > 1:1458551/1‑71 (MQ=255) ggTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAAccc < 1:2329952/64‑1 (MQ=255) ggTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCa < 1:2349975/71‑1 (MQ=255) ggTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCa < 1:406205/71‑1 (MQ=255) ggTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCa < 1:399146/71‑1 (MQ=255) ggTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCa < 1:3912208/71‑1 (MQ=255) gTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGt < 1:2644091/51‑1 (MQ=255) tAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTgg > 1:1006757/1‑48 (MQ=255) tAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTgg > 1:245269/1‑48 (MQ=255) tAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTgg > 1:422627/1‑48 (MQ=255) tAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTgg > 1:1578987/1‑48 (MQ=255) tAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTgg > 1:2654620/1‑48 (MQ=255) tAACTGTG‑CAACCAGACTTAGTTCGCAATCGACCAGCACGCTCTTgg > 1:1610706/1‑47 (MQ=38) tgCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATa < 1:934642/70‑1 (MQ=255) ccAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATaaa < 1:1280/70‑1 (MQ=255) ccAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATaaa < 1:3693852/70‑1 (MQ=255) ccAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATaaa < 1:2002873/70‑1 (MQ=255) cTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCa < 1:408558/51‑1 (MQ=255) tAGTTCGCTATCGACCAGCACGCTCTTGGTATGCAGTaa > 1:1276286/1‑39 (MQ=255) tAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTaa > 1:3861003/1‑39 (MQ=255) tAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTaa > 1:3726715/1‑39 (MQ=255) tAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTaa > 1:728617/1‑39 (MQ=255) tAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTa > 1:1774152/1‑38 (MQ=255) aGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCaaa > 1:435044/1‑50 (MQ=255) cATCGACCAGTACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccagc < 1:2214077/71‑1 (MQ=255) cATCGACCAGTACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccagc < 1:3523815/71‑1 (MQ=255) aTCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccag > 1:2661615/1‑69 (MQ=255) cACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGt > 1:1718473/1‑69 (MQ=255) | GAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGT > W3110S.gb/1309067‑1309204 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |