| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,336,529 | C→T | 32.3% | L321L (CTG→TTG) | cysB → | DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,336,529 | 0 | C | T | 32.3% | 41.2 / 26.8 | 34 | L321L (CTG→TTG) | cysB | DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding |
| Reads supporting (aligned to +/- strand): ref base C (11/12); new base T (8/3); total (19/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATAT > W3110S.gb/1336461‑1336595 | aTGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAATTg > 1:606800/1‑71 (MQ=255) tGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTg < 1:342663/70‑1 (MQ=255) gTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCgg > 1:1582981/1‑70 (MQ=255) tGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAATTGCCGGAAAaat < 1:1428693/71‑1 (MQ=255) gCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAAtt < 1:3387854/71‑1 (MQ=255) cTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAAttt > 1:1156688/1‑71 (MQ=255) gCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGc > 1:2657482/1‑71 (MQ=255) ttGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGtt < 1:1803679/71‑1 (MQ=255) ttGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGtt < 1:1953961/71‑1 (MQ=255) aTGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGc < 1:1503161/70‑1 (MQ=255) ttGAGGTCATGTTTAAAGATATAAAATTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATg > 1:1584315/1‑71 (MQ=255) gAGGTCATGTTTAAAGATATAAAATTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTa > 1:427022/1‑67 (MQ=255) gAGGTCATGTTTAAAGATATAAAATTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTa > 1:4059962/1‑67 (MQ=255) gAGGTCATGTTTAAAGATATAAAATTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTa > 1:1026796/1‑67 (MQ=255) gAGGTCATGTTTAAAGATATAAAATTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACATTTTa > 1:1655436/1‑67 (MQ=255) ggTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGc > 1:963652/1‑71 (MQ=255) tCATGTTTAAAGATATAAAACTGCCGGAAAAATAAttt < 1:2289761/38‑1 (MQ=255) aTGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTATCGaa < 1:1131376/70‑1 (MQ=255) aTGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGaa < 1:408097/70‑1 (MQ=255) aTGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAg > 1:498091/1‑71 (MQ=255) gTTTAAAGATATAAAATTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTa > 1:840073/1‑59 (MQ=255) tttAAAGATATAAAATTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCttt < 1:2411388/56‑1 (MQ=255) tttAAAGATATAAAATTGCCGGAAAAATAATTTCTTGCGTTATTTACGGCACCttt < 1:143539/56‑1 (MQ=255) tttAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGt < 1:3157589/70‑1 (MQ=255) aaaGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGaa < 1:718790/64‑1 (MQ=255) aaGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCgg < 1:3943949/71‑1 (MQ=255) atatAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCttt < 1:3066239/49‑1 (MQ=255) ataAAATTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAg > 1:162515/1‑59 (MQ=255) taAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCtttt > 1:2654601/1‑47 (MQ=255) taAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAatata > 1:1274478/1‑71 (MQ=255) aaaaCTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAg > 1:29962/1‑52 (MQ=255) aaaaCTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAatatat > 1:3525451/1‑71 (MQ=255) aaaaCTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAatata > 1:4061850/1‑70 (MQ=255) aaaCTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAg > 1:3418251/1‑51 (MQ=255) | ATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTGCCGGAAAAATAATTTCTTGCGTTATTTTCGGCACCTTTTATGTAGCGAAGGTGCCGGAATATAT > W3110S.gb/1336461‑1336595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |