| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,366,654 | T→C | 20.0% | Y237H (TAT→CAT) | puuB → | gamma‑Glu‑putrescine oxidase, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,366,654 | 0 | T | C | 20.0% | 49.1 / 8.6 | 25 | Y237H (TAT→CAT) | puuB | gamma‑Glu‑putrescine oxidase, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (14/6); new base C (3/2); total (17/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
AACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCAC > W3110S.gb/1366602‑1366721 | aaCTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGCATCTGGGCGATAAAGTag < 1:2499575/71‑1 (MQ=255) aaaGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGa < 1:2898022/71‑1 (MQ=255) ggTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACt > 1:2655050/1‑71 (MQ=255) ggTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACt > 1:187770/1‑71 (MQ=255) gTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGCATCTGGGCGATAAAg < 1:1852574/52‑1 (MQ=255) gTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGCATCTGGGCGATAAAGTAGAGCCGGAACTGGCGa > 1:921177/1‑70 (MQ=255) gTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGCATCTGGGCGATAAAGTAGAGCCGGAACTGGCGa > 1:2507828/1‑70 (MQ=255) gCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAATTAGAGCCGGAACTGGCGAAACgcag < 1:4016362/71‑1 (MQ=255) gCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACgcag < 1:478095/71‑1 (MQ=255) gAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTa > 1:3783332/1‑46 (MQ=255) aTGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTa > 1:1822206/1‑41 (MQ=255) aTGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTa > 1:1334450/1‑41 (MQ=255) aTGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTa > 1:3000424/1‑41 (MQ=255) aTGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTgg > 1:839926/1‑54 (MQ=255) aTGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTgg > 1:2682977/1‑54 (MQ=255) tgtgATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGaaa > 1:1763186/1‑58 (MQ=255) gtgATTGTCGCCGGGAATGCGCATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTg > 1:3823670/1‑71 (MQ=255) gATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGcc > 1:1923815/1‑66 (MQ=255) gATTGTCGCCGGCAATGCGTATCTGTGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGcc > 1:1408380/1‑66 (MQ=255) gCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGc < 1:2827982/70‑1 (MQ=255) gCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGc < 1:1149443/70‑1 (MQ=255) ggAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTg < 1:156057/71‑1 (MQ=255) cGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATcac > 1:3153934/1‑70 (MQ=255) cGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATcac > 1:679181/1‑70 (MQ=255) cGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATcac > 1:2011485/1‑70 (MQ=255) | AACTGCCAAAGGTCAGGTGACGGCGAAGTATGTGATTGTCGCCGGGAATGCGTATCTGGGCGATAAAGTAGAGCCGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCAC > W3110S.gb/1366602‑1366721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |