| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,367,159 | C→T | 31.4% | T405M (ACG→ATG) | puuB → | gamma‑Glu‑putrescine oxidase, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,367,159 | 0 | C | T | 31.4% | 44.4 / 26.8 | 35 | T405M (ACG→ATG) | puuB | gamma‑Glu‑putrescine oxidase, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (13/11); new base T (4/7); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
GGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATT > W3110S.gb/1367095‑1367228 | ggcgACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCATGCTGCg < 1:3470456/71‑1 (MQ=255)ggcgACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCATGCTGCg < 1:1923658/71‑1 (MQ=255)ggcgACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCATGCTGCg < 1:2155683/71‑1 (MQ=255) cgACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCATGCTGCg < 1:3183193/69‑1 (MQ=255) gAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCATGCTGCGTGTGCCGtt < 1:1629376/71‑1 (MQ=255) gAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGtt > 1:2895448/1‑71 (MQ=255) gATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGAt < 1:1805352/71‑1 (MQ=255) ccTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATggg < 1:312296/70‑1 (MQ=255) ccTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACCCTGCGTGTGCCGTTTACCGCGATggg < 1:3476612/70‑1 (MQ=255) cGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGtgtg > 1:1058980/1‑49 (MQ=255) cGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGtgtg > 1:1342549/1‑49 (MQ=255) cGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGtgtg > 1:328116/1‑49 (MQ=255) cGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGtgtg > 1:2823294/1‑49 (MQ=255) aaTCTGCCGCATTACCCGTTCCCCGGCGGGCGCATGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTa > 1:504407/1‑71 (MQ=255) aaTCTGCCGCATTACCCGTTCCCCGGCGGGCGCATGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTa > 1:1309489/1‑71 (MQ=255) tCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACctcg < 1:1109391/55‑1 (MQ=255) tGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTa > 1:492985/1‑70 (MQ=255) tACCCGTTCCCCGGCGGGCGCATGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTgcg > 1:2461269/1‑71 (MQ=255) tACCCGTTCCCCGGCGGGCGCATGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTgcg > 1:947549/1‑71 (MQ=255) aCCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTgc > 1:3073206/1‑69 (MQ=255) cccGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGcgcg < 1:615659/71‑1 (MQ=255) cccGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGAGGCGTATTACAGCCTgcgc > 1:909835/1‑70 (MQ=255) gTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATc > 1:924460/1‑71 (MQ=255) gTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATc > 1:861451/1‑71 (MQ=255) ttCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCg > 1:3226846/1‑71 (MQ=255) ttCCCCGGCGGGCGAATGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCg < 1:4083251/71‑1 (MQ=255) gggcgCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGt > 1:2638231/1‑71 (MQ=255) gggcgCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGt > 1:948925/1‑71 (MQ=255) gcgcATGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTAtt < 1:1612207/44‑1 (MQ=255) gcgcACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGttt < 1:1216162/71‑1 (MQ=255) gcgcACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGttt < 1:1843018/71‑1 (MQ=255) gcgcACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGttt < 1:1973478/71‑1 (MQ=255) gcgcACGCCGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGttt < 1:3327497/71‑1 (MQ=255) cgcACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGttt < 1:3818403/70‑1 (MQ=255) aCGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAAtt < 1:382679/71‑1 (MQ=255) | GGCGACGCCGAACGTTTCGATGCCTTCGCCAATCTGCCGCATTACCCGTTCCCCGGCGGGCGCACGCTGCGTGTGCCGTTTACCGCGATGGGCGCGGCGTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATT > W3110S.gb/1367095‑1367228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |