| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,413,273 | G→T | 23.1% | R130R (CGC→CGA) | ydaO ← | predicted C32 tRNA thiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,413,273 | 0 | G | T | 23.1% | 145.0 / 22.1 | 56 | R130R (CGC→CGA) | ydaO | predicted C32 tRNA thiolase |
| Reads supporting (aligned to +/- strand): ref base G (29/14); new base T (10/3); total (39/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCAC > W3110S.gb/1413203‑1413332 | tatCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcg > 1:1729536/1‑71 (MQ=255)tatCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcg > 1:1450464/1‑71 (MQ=255) tCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcgc < 1:3855314/70‑1 (MQ=255) tCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcgc < 1:2587062/70‑1 (MQ=255) tCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcgc < 1:1549009/70‑1 (MQ=255) cGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCTcga > 1:3733934/1‑71 (MQ=255) cGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATAACTcga > 1:780204/1‑71 (MQ=255) gTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCAAGTTCCGTTGCGGTACGATAAAGGATACCGcgac > 1:2726938/1‑71 (MQ=255) tCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgacg < 1:1187881/71‑1 (MQ=255) aCGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgacg > 1:632765/1‑69 (MQ=255) aCGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgacg > 1:3542533/1‑69 (MQ=255) ggTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcga > 1:629754/1‑62 (MQ=255) ggTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGc > 1:1875447/1‑69 (MQ=255) ggTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGc > 1:3914789/1‑69 (MQ=255) gTGACCCAACGCGATCTTCGTCGTCCCCAGTTCCGTTGCGGTACGATAAAGGATACCTCGACGAAGGCgag < 1:2666422/71‑1 (MQ=255) gTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGc > 1:854874/1‑68 (MQ=255) tGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCTCGACGAAGGCgag < 1:380640/70‑1 (MQ=255) cgATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCTCGACGAAGGCGAGAAcaca > 1:2620773/1‑66 (MQ=255) cgATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCTCGACGAAGGCGAGAAcaca > 1:3893952/1‑66 (MQ=255) cgATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCTCGACGAAGGCGAGAAcaca > 1:658742/1‑66 (MQ=255) ttCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGt > 1:3817437/1‑71 (MQ=255) cgtcgCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGc < 1:3108444/65‑1 (MQ=255) cgtcgCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAg < 1:3662855/68‑1 (MQ=255) ccccAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg < 1:3821216/70‑1 (MQ=255) ccccAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg < 1:411908/70‑1 (MQ=255) ccccAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg < 1:1706323/70‑1 (MQ=255) ccccAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg < 1:104023/70‑1 (MQ=255) ccccAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg < 1:1263469/70‑1 (MQ=255) gTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAg > 1:2534936/1‑37 (MQ=255) gTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCaa < 1:1457638/56‑1 (MQ=255) gTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCctct > 1:1919666/1‑71 (MQ=255) gTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCctct > 1:2604331/1‑71 (MQ=255) gTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGGGGTTTTGCCctct > 1:3690517/1‑71 (MQ=255) ttCCGTTGCGGTACGATAAAGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTg > 1:3101376/1‑71 (MQ=255) ttCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGc < 1:204836/53‑1 (MQ=255) cGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGa < 1:1514913/70‑1 (MQ=255) gCGGTACGATAAAGGATACCTCGACGAAGGCGAGAACACAg > 1:2734672/1‑41 (MQ=255) gCGGTACGATAAAGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtct < 1:1706524/70‑1 (MQ=255) gCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtctt > 1:1731217/1‑71 (MQ=255) gCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtctt > 1:1888908/1‑71 (MQ=255) gCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtctt > 1:3972704/1‑71 (MQ=255) ggTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtcttct > 1:3234633/1‑71 (MQ=255) tACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGc > 1:2999739/1‑54 (MQ=255) tACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGc > 1:2982866/1‑54 (MQ=255) tACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGc > 1:2508137/1‑54 (MQ=255) tACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGc > 1:1578277/1‑54 (MQ=255) aCGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCtt > 1:656976/1‑71 (MQ=255) aCGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCtt > 1:1917339/1‑71 (MQ=255) cGATAAAGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTGGttt > 1:307108/1‑49 (MQ=255) cGATAAAGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTGGttt > 1:2643647/1‑49 (MQ=255) cGATAAAGGATACCGGGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCttt > 1:290126/1‑71 (MQ=255) cGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCttt > 1:1416618/1‑71 (MQ=255) cGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCttt > 1:3556537/1‑71 (MQ=255) aTAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCa > 1:2229240/1‑71 (MQ=255) aTAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCa > 1:1929514/1‑71 (MQ=255) aTAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCa > 1:1555673/1‑71 (MQ=255) tAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCAc < 1:947189/71‑1 (MQ=255) tAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCAc < 1:3582913/71‑1 (MQ=255) tAAAGGATACCGAGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCAc < 1:3883197/71‑1 (MQ=255) aaaGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTATCAc > 1:2499392/1‑70 (MQ=255) aaaGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCAc > 1:3648369/1‑70 (MQ=255) | TATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCAC > W3110S.gb/1413203‑1413332 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |