| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,475,513 | A→G | 37.5% | pseudogene (8408/8622 nt) pseudogene (3110/3324 nt) |
ydbA → ydbA → |
ECK1398:JW5802+JW1402:b4492; predicted outer membrane protein ECK1398:JW1402:b1405; predicted outer membrane protein, C‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,475,513 | 0 | A | G | 37.5% | 8.7 / 12.6 | 16 | pseudogene (8408/8622 nt) pseudogene (3110/3324 nt) | ydbA ydbA | ECK1398:JW5802+JW1402:b4492; predicted outer membrane protein ECK1398:JW1402:b1405; predicted outer membrane protein, C‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base A (5/5); new base G (4/2); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCAGGTAAAGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTACGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTAACGTCGATGACGGT > W3110S.gb/1475454‑1475580 | cGCAGGTAAAGACGGCTGGAGAGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTACGCGAAGAGc > 1:954369/1‑70 (MQ=255) cAGGTAAAGACGGCTGGAGCGCTAGCGCTACGCTTGAAGGCGGGCCGAACCTGAGCTACGCGAAGAGCCAg < 1:2706953/71‑1 (MQ=255) gTAAAGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTAcgcg < 1:1497473/59‑1 (MQ=255) tAAAGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTACGCGAAGAGCCAGCGTa > 1:1058138/1‑71 (MQ=255) tAAAGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTACGCGAAGAGCCAGCGTa < 1:1462756/71‑1 (MQ=255) aaaGCCGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTAc > 1:1593636/1‑71 (MQ=255) aaaGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTAc > 1:1809955/1‑71 (MQ=255) aaaGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTAc > 1:1919134/1‑71 (MQ=255) aaaGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTACGCGAAGAGCCAGCGTAc > 1:2243020/1‑71 (MQ=255) aaaGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTACGCGAAGAGCCAGCGTAc > 1:3110025/1‑71 (MQ=255) gACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTGCGAGAAGAGCCAGCGTACGGc < 1:1125900/71‑1 (MQ=255) ctagcgctaCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGgcagg < 1:296191/70‑1 (MQ=255) aaGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCAc > 1:2253505/1‑71 (MQ=255) cGAACCTGAGCTACGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTAACGTc < 1:310363/71‑1 (MQ=255) cTGAGCTACGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTa < 1:1243465/61‑1 (MQ=255) tACGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTAACGTCTATGACGGt > 1:2379284/1‑69 (MQ=255) | CGCAGGTAAAGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTACGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTAACGTCGATGACGGT > W3110S.gb/1475454‑1475580 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |