Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,482,053	A→G	14.6%	D401G (GAT→GGT)	ynbC →	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,482,053	0	A	G	14.6%	96.5 / 10.7	41	D401G (GAT→GGT)	ynbC	predicted hydrolase
Reads supporting (aligned to +/- strand):  ref base A (26/9);  new base G (2/4);  total (28/13)
Fisher's exact test for biased strand distribution p-value = 6.85e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTATGTACT  >  W3110S.gb/1481984‑1482119                                                                      |                                                                   gtgtTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGAt                                                                   >  1:1994098/1‑71 (MQ=255)   gtTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGAtct                                                                 >  1:2542979/1‑71 (MQ=255)    ttGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGAtctc                                                                >  1:3438539/1‑71 (MQ=255)    ttGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGAtctc                                                                <  1:1212361/71‑1 (MQ=255)                     cGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAg                                                <  1:2674151/70‑1 (MQ=255)                     cGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAg                                                >  1:2293534/1‑70 (MQ=255)                       ccAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCaa                                                        <  1:3387945/60‑1 (MQ=255)                         aGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAg                                                      >  1:1894811/1‑60 (MQ=255)                         aGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAg                                                      >  1:180617/1‑60 (MQ=255)                         aGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAg                                                      >  1:3299694/1‑60 (MQ=255)                               aaaCCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTg                                     <  1:3136462/71‑1 (MQ=255)                                  cccATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGtt                                  >  1:3138226/1‑71 (MQ=255)                                    caattACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa                                <  1:466447/68‑1 (MQ=255)                                    cATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa                                <  1:2433381/71‑1 (MQ=255)                                     aTTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGAc                               <  1:2220974/71‑1 (MQ=255)                                      tttACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa                                <  1:1164313/69‑1 (MQ=255)                                        tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                            >  1:3537285/1‑71 (MQ=255)                                        tACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAg                                                      >  1:3783928/1‑45 (MQ=255)                                        tACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAg                                                      >  1:2952514/1‑45 (MQ=255)                                        tACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                            >  1:2284053/1‑71 (MQ=255)                                        tACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                            >  1:3113771/1‑71 (MQ=255)                                        tACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                            >  1:3451964/1‑71 (MQ=255)                                        tACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                            >  1:3109309/1‑71 (MQ=255)                                        tACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                            >  1:1893734/1‑71 (MQ=255)                                        tACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                            >  1:424905/1‑71 (MQ=255)                                        tACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                            >  1:478525/1‑71 (MQ=255)                                        tACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                            >  1:127438/1‑71 (MQ=255)                                        tACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                            >  1:1192312/1‑71 (MQ=255)                                        tACAAATACTGATTAAACAAGCCGTTGACGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                            >  1:670379/1‑71 (MQ=255)                                          cAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt                            <  1:871580/69‑1 (MQ=255)                                          cAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAt                             <  1:1425526/68‑1 (MQ=255)                                                   aTTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCa                 <  1:4030800/71‑1 (MQ=255)                                                    ttAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGa                                <  1:650704/55‑1 (MQ=255)                                                     tAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGTGCATg               >  1:3257980/1‑71 (MQ=255)                                                     tAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATg               >  1:1713107/1‑71 (MQ=255)                                                            gCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCTTGGTTGACATTGCCGCAGGGCATGGGCGCTa        >  1:2858143/1‑71 (MQ=255)                                                            gCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTa        >  1:1921874/1‑71 (MQ=255)                                                            gCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTa        >  1:635770/1‑71 (MQ=255)                                                               gTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAt                             <  1:1625238/47‑1 (MQ=255)                                                                  gCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTATGTACt  >  1:1536947/1‑71 (MQ=255)                                                                  gCCGATCTCCACGCCAAAGGTTTAGCCGTCCGAGTGGTTGACATTGCCGCAGGGCATGGGCGCTATGTACt  >  1:1669361/1‑71 (MQ=255)                                                                      |                                                                   GTGTTGGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGCTATGTACT  >  W3110S.gb/1481984‑1482119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑