| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,487,609 | C→T | 21.4% | R926R (CGC→CGT) | hrpA → | ATP‑dependent helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,487,609 | 0 | C | T | 21.4% | 58.1 / 9.0 | 28 | R926R (CGC→CGT) | hrpA | ATP‑dependent helicase |
| Reads supporting (aligned to +/- strand): ref base C (13/9); new base T (2/4); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGAT > W3110S.gb/1487541‑1487675 | aaCTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGTGa < 1:2512866/71‑1 (MQ=255) tATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGcgcg < 1:2210138/67‑1 (MQ=255) tGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTAc > 1:3425298/1‑71 (MQ=255) tttAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGTGAGTTACGGCGGATGACCg > 1:3751117/1‑71 (MQ=255) gcgTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACCGCGGATGACCGGCGTTAcc < 1:2227723/71‑1 (MQ=255) tCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGtt < 1:3853693/71‑1 (MQ=255) tCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGtt < 1:3338847/71‑1 (MQ=255) aaaCCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGTGAGTTACGGCGGATGACCGGCGTTACCGTTGa > 1:4000261/1‑71 (MQ=255) aaaCCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAg > 1:2410873/1‑42 (MQ=255) aaaCCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGa > 1:1882918/1‑71 (MQ=255) aGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGAc < 1:1541626/71‑1 (MQ=255) aGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGAc < 1:174461/71‑1 (MQ=255) gTTACCGTTGCTCGAGAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGAca > 1:2704712/1‑70 (MQ=255) gTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGa > 1:1817463/1‑69 (MQ=255) gTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACt > 1:3191834/1‑71 (MQ=255) gTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACt > 1:3205247/1‑71 (MQ=255) aCCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTAc > 1:2907293/1‑51 (MQ=255) aCCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTAc > 1:314488/1‑51 (MQ=255) aCCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTAc > 1:2418691/1‑51 (MQ=255) aCCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTgg > 1:3016265/1‑70 (MQ=255) aCCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTgg > 1:3388766/1‑70 (MQ=255) ccGTTGCTCGACAGCCTTGAGCGTGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGc < 1:2866571/70‑1 (MQ=255) ccGTTGCTCGACAGCCTTGAGCGTGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGc < 1:3370547/70‑1 (MQ=255) ccGTTGCTAGACAGCCTTGAGCGTGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGc < 1:330618/70‑1 (MQ=255) gACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATc < 1:49162/70‑1 (MQ=255) gACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCa < 1:190701/71‑1 (MQ=255) gAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCg > 1:491976/1‑70 (MQ=255) gAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCg < 1:1630391/70‑1 (MQ=255) aGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGAt < 1:3250747/71‑1 (MQ=255) | AACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTCGACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGGCACTGGGATCAGGTGCCCGAT > W3110S.gb/1487541‑1487675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |