| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,489,179 | T→C | 26.1% | F77F (TTT→TTC) | ydcF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,489,179 | 0 | T | C | 26.1% | 108.6 / 44.3 | 65 | F77F (TTT→TTC) | ydcF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (30/18); new base C (6/11); total (36/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.72e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGGCAGAGCAGAAGCGAC > W3110S.gb/1489111‑1489247 | tGTAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACtttctt < 1:3431195/71‑1 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg < 1:2689795/70‑1 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg > 1:1143130/1‑70 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg < 1:2949491/70‑1 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg > 1:652602/1‑70 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg > 1:1658524/1‑70 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg > 1:1687337/1‑70 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg > 1:3645999/1‑70 (MQ=255) tAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTCTTg < 1:3478154/70‑1 (MQ=255) aaGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTg < 1:232408/69‑1 (MQ=255) aGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGta < 1:2427592/70‑1 (MQ=255) aGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGta > 1:2131250/1‑70 (MQ=255) aGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGta < 1:3583887/70‑1 (MQ=255) aTTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGta > 1:1865964/1‑68 (MQ=255) tGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGta > 1:805062/1‑66 (MQ=255) tGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGta > 1:1242079/1‑66 (MQ=255) gcgATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATc > 1:2138132/1‑71 (MQ=255) gcgATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATc > 1:1253376/1‑71 (MQ=255) gcgATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATc > 1:2950373/1‑71 (MQ=255) gcgATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGta > 1:596492/1‑61 (MQ=255) cgATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGTATAGCGCCATCg < 1:2038703/71‑1 (MQ=255) cgATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTCTTGTATAGCCCCATCg < 1:685586/71‑1 (MQ=255) aTCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTCTTg < 1:3342378/56‑1 (MQ=255) cAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGta > 1:3472826/1‑56 (MQ=255) cAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGta > 1:973918/1‑56 (MQ=255) aGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAgcgc < 1:194338/61‑1 (MQ=255) cAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCa > 1:3101191/1‑71 (MQ=255) ttCCTTTACTGATTAGTGGTGATATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCg > 1:2090606/1‑71 (MQ=255) tAGTGGTGGTATCGGTCACTCGACAACTTTCTTGta > 1:2890957/1‑36 (MQ=255) tAGTGGTGGTATCGGTCACTCGACAACTTTCTTGta > 1:2393120/1‑36 (MQ=255) aGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTa > 1:774910/1‑70 (MQ=255) gtggtATCGGTCACTCGACAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGc < 1:347287/71‑1 (MQ=255) gtggtATCGGTCACTCGACAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGc < 1:3542045/71‑1 (MQ=255) tCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCg < 1:250482/64‑1 (MQ=255) tCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCg < 1:3953262/64‑1 (MQ=255) tCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCg < 1:1687575/64‑1 (MQ=255) tCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCg < 1:1595403/64‑1 (MQ=255) tCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACt > 1:1866500/1‑71 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATc > 1:3757362/1‑43 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATc > 1:3222120/1‑43 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATc > 1:3253978/1‑43 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTgg > 1:3877931/1‑71 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTgg < 1:3556830/71‑1 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTgg > 1:2485231/1‑71 (MQ=255) ggTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTgg > 1:3927350/1‑71 (MQ=255) cACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTac < 1:3256053/48‑1 (MQ=255) aCTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACt < 1:3140485/53‑1 (MQ=255) aCTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGgcag < 1:3211297/70‑1 (MQ=255) aCTCGACAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTAt > 1:3314805/1‑55 (MQ=255) cTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACt < 1:3695178/44‑1 (MQ=255) cTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACt < 1:2779186/44‑1 (MQ=255) cTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACt < 1:3205438/44‑1 (MQ=255) cTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTacaa > 1:1324102/1‑48 (MQ=255) cTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTacaa > 1:4074206/1‑48 (MQ=255) cTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTacaa > 1:3043874/1‑48 (MQ=255) cTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTacaa > 1:312957/1‑48 (MQ=255) cGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGgcagag > 1:3063864/1‑69 (MQ=255) gACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGgcagag > 1:1116668/1‑68 (MQ=255) gACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGgcagag > 1:1706078/1‑68 (MQ=255) gACAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGgcagag > 1:3068936/1‑68 (MQ=255) aCAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACt < 1:3079251/60‑1 (MQ=255) aCAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGgcagagcag < 1:465958/70‑1 (MQ=255) aCAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGgcagagcag < 1:2233719/70‑1 (MQ=255) aCAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGgcagagcag < 1:1353022/70‑1 (MQ=255) aCAACTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGgcagagcag < 1:1114893/70‑1 (MQ=255) aaCTTTCTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGgcagagcaga < 1:1851413/69‑1 (MQ=255) tttttGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGGCAGAGCAGAAGCGAc > 1:3517541/1‑71 (MQ=255) tttttGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGGCAGAGCAGAAGCGAc > 1:425561/1‑71 (MQ=255) ttttGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGGCAGAGCAGAAGCGAc < 1:550100/70‑1 (MQ=255) ttttGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGGCAGAGCAGAAGCGAc < 1:3340535/70‑1 (MQ=255) | TGTAAGATTGCCCGCGATCAGCAAATTCCTTTACTGATTAGTGGTGGTATCGGTCACTCGACAACTTTTTTGTATAGCGCCATCGCACAGCATCCGCACTACAACACTATCCGCACCACTGGCAGAGCAGAAGCGAC > W3110S.gb/1489111‑1489247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |