Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 1,507,250 | A→G | 21.9% | I182T (ATC→ACC) | ydcO ← | predicted benzoate transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,507,250 | 0 | A | G | 21.9% | 165.4 / 45.6 | 82 | I182T (ATC→ACC) | ydcO | predicted benzoate transporter |
Reads supporting (aligned to +/- strand): ref base A (44/20); new base G (14/4); total (58/24) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.66e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTT > W3110S.gb/1507181‑1507315 | tCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGAt < 1:343455/71‑1 (MQ=255) atatAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGGTGacgatcac < 1:3896686/71‑1 (MQ=255) atatAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGGTGacgatcac < 1:3646752/71‑1 (MQ=255) atatAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGacgatcac > 1:2539158/1‑71 (MQ=255) atatAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGacgatcac > 1:1278281/1‑71 (MQ=255) atatAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGacgatcac > 1:260303/1‑71 (MQ=255) atatAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGacgatcac > 1:3606781/1‑71 (MQ=255) tataAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGGTGacg < 1:1118899/65‑1 (MQ=255) ataAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGacgatcacga > 1:160476/1‑71 (MQ=255) ataAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGacgatcacga > 1:3005231/1‑71 (MQ=255) ataAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGacgatcacga > 1:1371220/1‑71 (MQ=255) taAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGacgatcacgat > 1:2435965/1‑71 (MQ=255) gagaACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCaataa < 1:3511245/71‑1 (MQ=255) gagaACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCaataa < 1:389204/71‑1 (MQ=255) gagaACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCaataa < 1:2370622/71‑1 (MQ=255) cGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAatcatc > 1:1789898/1‑71 (MQ=255) ttAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCgg > 1:2047840/1‑70 (MQ=255) tAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGc < 1:82978/70‑1 (MQ=255) tAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCaataat > 1:950562/1‑61 (MQ=255) tAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCaataat > 1:346023/1‑61 (MQ=255) tAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCgg > 1:2579881/1‑69 (MQ=255) tAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCgg > 1:2593402/1‑69 (MQ=255) tAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGc < 1:2718855/70‑1 (MQ=255) tAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGc < 1:579093/70‑1 (MQ=255) tAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGc > 1:1628420/1‑70 (MQ=255) aaaGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCa < 1:2786816/70‑1 (MQ=255) aaGACAACATCAGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCaa > 1:2608898/1‑70 (MQ=255) acaacaTCAGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCAATTAc > 1:3877240/1‑71 (MQ=255) acaacaTCAGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCAATTAc > 1:364686/1‑71 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCaa > 1:1091012/1‑65 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCaa > 1:2332547/1‑65 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCaa > 1:3996331/1‑65 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCaa > 1:1204942/1‑65 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGAT‑CCAATAATCATCGCGGCaa > 1:2916143/1‑64 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATcc < 1:2687425/47‑1 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATcc < 1:3814340/47‑1 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCg > 1:4021915/1‑71 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCg < 1:425641/71‑1 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCg > 1:660937/1‑71 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCg > 1:1735137/1‑71 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCg > 1:2551298/1‑71 (MQ=255) acaTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGc < 1:2079300/71‑1 (MQ=255) aTCAGTTGTGACAACGTCACCTTGCGCTATGACGATCACGATCCCAATAATCATCGCGGCaa > 1:3472277/1‑62 (MQ=255) aTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCaa > 1:1949732/1‑62 (MQ=255) aTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCaa > 1:1294286/1‑62 (MQ=255) tCAGTTGTGACAACGTCACCTTGCGCGATGacgatca > 1:2064556/1‑37 (MQ=255) tCAGTTGTGACAACGTCACCTTGCGCGATGacgatca > 1:2358851/1‑37 (MQ=255) tCAGTTGTGACAACGTCACCTTGCGCGATGacgatca > 1:3030749/1‑37 (MQ=255) tCAGTTGTGACAACGTCACCTTGCGCGATGacgatc > 1:3765904/1‑36 (MQ=255) tCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCaata < 1:2682096/48‑1 (MQ=255) tCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCaata < 1:931475/48‑1 (MQ=255) tCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCaata < 1:331247/48‑1 (MQ=255) tCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAatca < 1:3534107/52‑1 (MQ=255) tCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAatca < 1:4037962/52‑1 (MQ=255) tCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAatca < 1:3529444/52‑1 (MQ=255) tCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAatca < 1:2818216/52‑1 (MQ=255) cAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAatcat > 1:514296/1‑52 (MQ=255) cAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAatcat > 1:1314246/1‑52 (MQ=255) aGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCAt > 1:512715/1‑68 (MQ=255) aGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCAt > 1:3361664/1‑68 (MQ=255) aGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAatcatc > 1:2651722/1‑52 (MQ=255) aGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAatcatc > 1:1878728/1‑52 (MQ=255) aGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAatcatc > 1:1846759/1‑52 (MQ=255) ttGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCaataat < 1:1569425/46‑1 (MQ=255) tgtgACAACGTCACCTTGCCCGATGACGATCACGATCCCAATAatca > 1:3853361/1‑47 (MQ=255) gtgACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGcgcg > 1:2598085/1‑70 (MQ=255) gtgACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCgcgg > 1:1476006/1‑71 (MQ=255) gtgACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCgcgg > 1:3157997/1‑71 (MQ=255) gtgACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCgcgg > 1:3924443/1‑71 (MQ=255) gtgACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCgcgg > 1:405176/1‑71 (MQ=255) gtgACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCgcgg > 1:2132210/1‑71 (MQ=255) aCCTTGCGCGATGACGATCACGATCCCAATAATCATCGCgg > 1:247002/1‑41 (MQ=255) aCCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGc > 1:1168447/1‑51 (MQ=255) aCCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCa > 1:2086870/1‑52 (MQ=255) aCCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCa > 1:3507716/1‑52 (MQ=255) aCCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCa > 1:3213885/1‑52 (MQ=255) aCCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCa > 1:1774738/1‑52 (MQ=255) aCCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCt > 1:2388825/1‑71 (MQ=255) aCCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCt > 1:3570402/1‑71 (MQ=255) aCCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCt > 1:2216671/1‑71 (MQ=255) aCCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCt > 1:2665109/1‑71 (MQ=255) cgcgATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGtt < 1:3626362/70‑1 (MQ=255) | TCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTT > W3110S.gb/1507181‑1507315 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |