Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,381,930 Δ1 bp 100% coding (333/759 nt) yfbB ← predicted peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,381,9300C.92.9% 42.5 / ‑1.3 14coding (333/759 nt)yfbBpredicted peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base . (6/7);  total (6/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

TTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGC  >  W3110S.gb/2381873‑2381980
                                                         |                                                  
ttGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAAt                                      <  1:1270845/71‑1 (MQ=255)
ttGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAAt                                      <  1:1699010/71‑1 (MQ=255)
ttGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAAt                                      <  1:688811/71‑1 (MQ=255)
ttGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGACG‑CCGCCTTCGACAAt                                      <  1:1478837/71‑1 (MQ=255)
ttGGCGATCGGAACGCTGACGTTCCGCACGTTGTTAAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACaa                                       <  1:3033880/71‑1 (MQ=255)
  ggCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATaa                                    >  1:1384247/1‑71 (MQ=255)
  ggCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATaa                                    >  1:2287789/1‑71 (MQ=255)
  ggCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATaa                                    >  1:3642062/1‑71 (MQ=255)
   gCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAAc                                   <  1:3198127/71‑1 (MQ=255)
        cGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATaa                                    >  1:620108/1‑65 (MQ=255)
        cGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATa                                     >  1:1669794/1‑64 (MQ=255)
            aCGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAg                          <  1:2194780/71‑1 (MQ=255)
            aCGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAg                          <  1:3203632/71‑1 (MQ=255)
                                        ttttGCAGCCCCGGATG‑CCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAgccgc  >  1:1525001/1‑67 (MQ=255)
                                                         |                                                  
TTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGC  >  W3110S.gb/2381873‑2381980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑