| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,512,687 | Δ1 bp | 100% | coding (654/1248 nt) | ypdG ← | predicted enzyme IIC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,512,687 | 0 | G | . | 96.7% | 111.0 / ‑1.6 | 30 | coding (654/1248 nt) | ypdG | predicted enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (16/13); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
CAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGG > W3110S.gb/2512621‑2512754 | cAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCTAGCCACCAAAggggt > 1:2279141/1‑70 (MQ=255)cAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAggggt > 1:3438440/1‑70 (MQ=255) cTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACg > 1:2483004/1‑69 (MQ=255) ctcACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAgg > 1:3683605/1‑56 (MQ=255) ctcACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAgg > 1:2247557/1‑56 (MQ=255) ctcACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACgtagta > 1:1836771/1‑71 (MQ=255) ctcACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACgtag < 1:1564492/69‑1 (MQ=255) gCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGc < 1:2606124/71‑1 (MQ=255) gCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGc < 1:239542/71‑1 (MQ=255) gTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAggggt < 1:897357/46‑2 (MQ=255) gTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAggggt < 1:2731816/46‑2 (MQ=255) gTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAggggt < 1:2332786/46‑2 (MQ=255) gTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAggggt < 1:2247512/46‑2 (MQ=255) gTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAggggt < 1:1837145/46‑2 (MQ=255) tCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAggggt < 1:1687749/45‑2 (MQ=255) tCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAgg < 1:697284/42‑1 (MQ=255) tCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAgg < 1:2007574/42‑1 (MQ=255) tCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAgg < 1:1434119/42‑1 (MQ=255) tCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAgg < 1:2463058/42‑1 (MQ=255) cAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAgg < 1:2655955/41‑1 (MQ=255) cAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAgg < 1:2467377/41‑1 (MQ=255) cAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGa > 1:1731060/1‑70 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGc > 1:1063488/1‑61 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGc > 1:2972631/1‑61 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGc > 1:1203000/1‑61 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGc > 1:2685224/1‑61 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGAc > 1:2362592/1‑70 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGAc > 1:1548488/1‑70 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGAc > 1:1336112/1‑70 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGAc < 1:1678726/70‑1 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGAc < 1:2246983/70‑1 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGAc > 1:2174688/1‑70 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGAc > 1:1147000/1‑70 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGAc > 1:3032005/1‑70 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGAc > 1:3008667/1‑70 (MQ=255) aGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGAc > 1:1879440/1‑70 (MQ=255) cGGATACCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCg < 1:1857116/71‑1 (MQ=255) aCCGCCGTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGa > 1:1780703/1‑68 (MQ=255) ccgccgTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGa < 1:3441242/71‑1 (MQ=255) ccgTTGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGc < 1:2876958/47‑1 (MQ=255) ttGATCCAGCCACCAAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACaaa < 1:2961677/52‑1 (MQ=255) ccaAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGa < 1:2892870/53‑1 (MQ=255) ccaAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGa < 1:113517/53‑1 (MQ=255) ccaAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGa < 1:375158/53‑1 (MQ=255) ccaAA‑GGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCaaa < 1:13248/65‑1 (MQ=255) aaaGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTgg > 1:1248241/1‑71 (MQ=255) | CAGCGCGCCCTTCTCACCTGCGGCAGTCAGCACAGTACGGATACCGCCGTTGATCCAGCCACCAAAGGGGGTGATGACGTAGTACATCGCCAGCATGACAAAAATGGCGGAAAGAATCGGGAGCAAAAATGTGG > W3110S.gb/2512621‑2512754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |