| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,792,107 | Δ1 bp | 100% | coding (315/1320 nt) | uhpC → | membrane protein regulates uhpT expression |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,792,107 | 0 | G | . | 75.9% | 100.3 / 62.9 | 58 | coding (315/1320 nt) | uhpC | membrane protein regulates uhpT expression |
| Reads supporting (aligned to +/- strand): ref base G (3/11); new base . (28/16); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01 | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
TGTCTCCGGCATTGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGCTCTGGGTG > W3110S.gb/3792041‑3792170 | tGTCTCCGGCATTGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACggcat > 1:2734921/1‑68 (MQ=255)tGTCTCCGGCATTGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACggcat > 1:2847536/1‑68 (MQ=255)tGTCTCCGGCATTGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACggcat > 1:3265792/1‑68 (MQ=255)tGTCTCCGGCATTGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACggcat > 1:3345236/1‑68 (MQ=255) tctcCGGCATTGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCAt < 1:920260/70‑1 (MQ=255) tctcCGGCATTGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCAt < 1:2406156/70‑1 (MQ=255) cGGCATTGTCAGCGATCGCTCATATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCaa < 1:305566/71‑1 (MQ=255) cGGCATTGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCaa < 1:2516404/71‑1 (MQ=255) ttGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCAt < 1:1525164/61‑1 (MQ=255) ttGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCAt < 1:3652222/61‑1 (MQ=255) ttGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCAt < 1:2930982/61‑1 (MQ=255) ttGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCAt < 1:3596194/61‑1 (MQ=255) ttGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCAt < 1:194431/61‑1 (MQ=255) gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCATTATCAACATTCt > 1:1089269/1‑71 (MQ=255) gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCt > 1:264149/1‑70 (MQ=255) gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:749584/1‑71 (MQ=255) gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:2668314/1‑71 (MQ=255) gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:888718/1‑71 (MQ=255) gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:231742/1‑71 (MQ=255) gTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTg > 1:1271546/1‑71 (MQ=255) aGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGttt < 1:123472/71‑1 (MQ=255) aGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGttt < 1:637110/71‑1 (MQ=255) tCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCATTATCAACATTCTGTTTGGCt < 1:1855984/71‑1 (MQ=255) cGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTtc > 1:2479014/1‑71 (MQ=255) cGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTtc > 1:3444629/1‑71 (MQ=255) gCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTtct > 1:3075151/1‑71 (MQ=255) gCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTtct > 1:784980/1‑71 (MQ=255) gCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTtct > 1:969914/1‑71 (MQ=255) cTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTtctc < 1:3249429/71‑1 (MQ=255) cTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTtctc < 1:347688/71‑1 (MQ=255) tCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACAt < 1:1920034/55‑1 (MQ=255) aaaTGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGa < 1:1448965/70‑1 (MQ=255) aaaTGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGa < 1:1628591/70‑1 (MQ=255) aaaTGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGa < 1:587571/70‑1 (MQ=255) aaaTGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGa < 1:583303/70‑1 (MQ=255) aaaTGCCCGTTATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGAc < 1:1637081/71‑1 (MQ=255) cGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCt < 1:1293773/71‑1 (MQ=255) ttATTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATg > 1:2028979/1‑71 (MQ=255) aTTTTATGGGGATAGGGCTTATCGCCACGGGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTa > 1:100089/1‑68 (MQ=255) aTTTTATGGGGATAGGGCTTATCGCCACGGGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTa > 1:491014/1‑68 (MQ=255) aTTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGc > 1:820458/1‑52 (MQ=255) aTTTTATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGc > 1:932832/1‑52 (MQ=255) ttATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATg > 1:1196698/1‑66 (MQ=255) ttATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATg > 1:655302/1‑66 (MQ=255) ttATGGGGATAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCg < 1:775463/70‑1 (MQ=255) tGGGGATAGGGCTTATCGCCACGGGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGtt < 1:943175/70‑1 (MQ=255) tGGGGATAGGGCTTATCGCCACGGGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGtt < 1:2116725/70‑1 (MQ=255) tAGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTtctc < 1:75856/45‑1 (MQ=38) aGGGCTTTTCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCg > 1:2964771/1‑67 (MQ=255) aGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCg > 1:2404318/1‑67 (MQ=255) aGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCg > 1:3542482/1‑67 (MQ=255) aGGGCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGct > 1:3422890/1‑71 (MQ=255) gggCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGctc > 1:2316622/1‑71 (MQ=255) gggCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGctc > 1:1069861/1‑71 (MQ=255) gggCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGctc > 1:901297/1‑71 (MQ=255) gggCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGct > 1:2321528/1‑70 (MQ=255) ggCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGctct < 1:2667075/71‑1 (MQ=255) ggCTTATCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGctcg < 1:3474684/71‑2 (MQ=255) aTCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGCTCTGGGt > 1:268359/1‑70 (MQ=255) aTCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGCTCTGGGTg > 1:887345/1‑71 (MQ=255) aTCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGCTCTGGGTg > 1:550918/1‑71 (MQ=255) aTCGCCAC‑GGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGCTCTGGGTg > 1:488042/1‑71 (MQ=255) | TGTCTCCGGCATTGTCAGCGATCGCTCAAATGCCCGTTATTTTATGGGGATAGGGCTTATCGCCACGGGCATTATCAACATTCTGTTTGGCTTCTCGACGTCGCTATGGGCGTTTGCCGTGCTCTGGGTG > W3110S.gb/3792041‑3792170 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |