| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,371,273 | Δ2 bp | 100% | intergenic (‑6/+299) | dcuA ← / ← aspA | C4‑dicarboxylate antiporter/aspartate ammonia‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,371,273 | 0 | A | . | 100.0% | 102.2 / NA | 28 | intergenic (‑6/+300) | dcuA/aspA | C4‑dicarboxylate antiporter/aspartate ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (8/20); total (8/20) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | W3110S.gb | 4,371,274 | 0 | A | . | 100.0% | 102.2 / NA | 28 | intergenic (‑7/+299) | dcuA/aspA | C4‑dicarboxylate antiporter/aspartate ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (8/20); total (8/20) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTT > W3110S.gb/4371203‑4371335 || gggTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGa < 1:2278304/71‑1 (MQ=255) ggTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGa < 1:680389/70‑1 (MQ=255) ttACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaa > 1:3320143/1‑70 (MQ=255) gcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATa < 1:2005504/71‑1 (MQ=255) cTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATa < 1:1806141/67‑1 (MQ=255) tgtctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGAt < 1:1147494/60‑1 (MQ=255) tctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGAt < 1:1117866/70‑1 (MQ=255) tgatgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa > 1:689006/1‑70 (MQ=255) atgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa < 1:817202/68‑1 (MQ=255) atgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa < 1:1319341/68‑1 (MQ=255) atgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa < 1:1793789/68‑1 (MQ=255) gaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATg > 1:1641961/1‑68 (MQ=255) aCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGAt < 1:3420777/71‑1 (MQ=255) cAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa < 1:3530023/71‑1 (MQ=255) ccAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGtt < 1:984979/71‑1 (MQ=255) ccAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGtt < 1:2525080/71‑1 (MQ=255) aGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGc < 1:2429257/36‑1 (MQ=25) aGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGc < 1:3665886/36‑1 (MQ=25) cAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa > 1:2913330/1‑36 (MQ=25) cAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa > 1:1478502/1‑36 (MQ=25) cAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa > 1:1443148/1‑36 (MQ=25) cAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCa > 1:119272/1‑36 (MQ=25) aaCCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGga > 1:1600595/1‑45 (MQ=38) aaCCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGga > 1:433719/1‑45 (MQ=38) tGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa < 1:3195189/46‑1 (MQ=37) tGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa < 1:3634528/46‑1 (MQ=37) tGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa < 1:544380/46‑1 (MQ=37) tGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa < 1:1013954/46‑1 (MQ=37) gCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa < 1:2294721/45‑1 (MQ=37) cATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGttt < 1:2244089/69‑1 (MQ=255) cATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGttt < 1:1498940/69‑1 (MQ=255) || GGGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTT > W3110S.gb/4371203‑4371335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |