| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,846,634 | Δ1 bp | 100% | intergenic (‑49/+79) | ynjI ← / ← topB | predicted inner membrane protein/DNA topoisomerase III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,846,634 | 0 | A | . | 94.7% | 67.0 / ‑1.9 | 19 | intergenic (‑49/+79) | ynjI/topB | predicted inner membrane protein/DNA topoisomerase III |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (6/12); total (6/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
TTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCG > W3110S.gb/1846565‑1846689 | ttGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCa > 1:2802874/1‑70 (MQ=255) taataaAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaaaa < 1:2658044/71‑1 (MQ=255) cTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCa < 1:2891231/71‑1 (MQ=255) cTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCa < 1:753187/71‑1 (MQ=255) tttttCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaaaaaat > 1:1565780/1‑63 (MQ=255) ttCATCACAATCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCaaaaa < 1:225688/58‑1 (MQ=255) acaTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCt < 1:1564193/71‑1 (MQ=255) acaTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCt > 1:2390633/1‑71 (MQ=255) aTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTg < 1:3720601/71‑1 (MQ=255) aTTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTg < 1:1227671/71‑1 (MQ=255) gTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCt > 1:1972788/1‑71 (MQ=255) gTTATTACATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCt > 1:1964078/1‑71 (MQ=255) attaCATACTTTAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGa > 1:1987979/1‑71 (MQ=255) ttAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa > 1:189329/1‑71 (MQ=255) ttAGAGGCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa > 1:3631042/1‑71 (MQ=255) agagGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGaa < 1:1718080/70‑1 (MQ=255) gCGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTg < 1:3036447/53‑1 (MQ=255) cGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAg < 1:1777192/62‑1 (MQ=255) cGATAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAg < 1:1409605/62‑1 (MQ=255) aTAATTTTATCCATGC‑AAAAAAATATCCAACGAAt < 1:3637513/35‑1 (MQ=38) tAATTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCg < 1:773662/70‑1 (MQ=255) aTTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGa < 1:2431685/40‑1 (MQ=39) aTTTTATCCATGC‑AAAAAAATATCCAACGAATTTTCTTGa < 1:2354491/40‑1 (MQ=39) | TTGTAATAAAACTTTTTTCATCACATTCCCTGTTATTACATACTTTAGAGGCGATAATTTTATCCATGCAAAAAAAATATCCAACGAATTTTCTTGATCCGCTGGATATTTCAGAATATGACTCG > W3110S.gb/1846565‑1846689 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |