| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,071,419:1 | +A | 100% | coding (331/1296 nt) | glgC → | glucose‑1‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,071,419 | 1 | . | A | 90.0% | 130.6 / 11.5 | 50 | coding (331/1296 nt) | glgC | glucose‑1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (5/0); new base A (29/16); total (34/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑AAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACATTATCCGCCGTTATAAAGCG > W3110S.gb/4071352‑4071487 | gTCATTCTTCAATGAAGAAATGAACGGGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑a < 1:2583206/69‑1 (MQ=255)gTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑aaa < 1:605678/71‑1 (MQ=255)gTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑aaa < 1:351464/71‑1 (MQ=255)gTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑aaa < 1:2964512/71‑1 (MQ=255)gTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑a < 1:386236/69‑1 (MQ=255) aTTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑AAAACt > 1:47332/1‑71 (MQ=255) ttcttcAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑aaaaact < 1:1596446/71‑4 (MQ=255) cttcAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑AAAACTgg > 1:3224830/1‑70 (MQ=255) cAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGt < 1:3130129/69‑1 (MQ=255) cAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGt < 1:348162/69‑1 (MQ=255) aagaaATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCgg > 1:2935867/1‑71 (MQ=255) tGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑aaa < 1:1453123/51‑1 (MQ=255) tGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑aaa < 1:2082924/51‑1 (MQ=255) tGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑aaa < 1:2737153/51‑1 (MQ=255) tGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑aaa < 1:1765839/51‑1 (MQ=255) tGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑aaa < 1:1863452/51‑1 (MQ=255) tGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑aaa < 1:3695574/51‑1 (MQ=255) tGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑aaa < 1:241072/51‑1 (MQ=255) gAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAGAGGGG‑aaaaa > 1:2270599/1‑51 (MQ=255) gAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCg > 1:3645433/1‑69 (MQ=255) aaCGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCa < 1:2008359/70‑1 (MQ=255) aaCGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑aaaaa > 1:2954255/1‑50 (MQ=255) aaCGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑aaaaa > 1:519829/1‑50 (MQ=255) aCGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCa < 1:1064493/69‑1 (MQ=255) tttGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCa < 1:145501/64‑1 (MQ=255) tGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGt > 1:1708786/1‑70 (MQ=255) tGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGt > 1:1458869/1‑70 (MQ=255) tGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGt > 1:327557/1‑70 (MQ=255) tGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGt > 1:834050/1‑70 (MQ=255) tGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGt > 1:1699105/1‑70 (MQ=255) tGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGt > 1:191107/1‑70 (MQ=255) tGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGt > 1:250624/1‑70 (MQ=255) tGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGt > 1:818915/1‑70 (MQ=255) tGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGt > 1:2145721/1‑70 (MQ=255) tGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGt > 1:126250/1‑70 (MQ=255) tGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGt < 1:1238090/70‑1 (MQ=255) gTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTTGTATCGCGGCACCGCAGATGCGGTc < 1:3508772/70‑1 (MQ=255) tCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGt < 1:3098740/68‑1 (MQ=255) ctgctgCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCg > 1:901658/1‑61 (MQ=255) ctgctgCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCg > 1:2957039/1‑61 (MQ=255) ctgctgCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCg > 1:1459763/1‑61 (MQ=255) ctgctgCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCaa > 1:3622083/1‑70 (MQ=255) ctgctgCCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCaa > 1:2770404/1‑70 (MQ=255) ctgctgCCAGCACAGCAGAGAATGAAAGGGG‑aaaa > 1:1351466/1‑35 (MQ=255) ctgcCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCaaa < 1:724658/68‑1 (MQ=255) ctgcCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCaaa < 1:1957694/68‑1 (MQ=255) ctgcCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCaaa < 1:3175344/68‑1 (MQ=255) ctgcCAGCACAGCAGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCaaa < 1:2019892/68‑1 (MQ=255) agcacagcaGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGa > 1:482140/1‑49 (MQ=38) cacagcaGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACAt < 1:2136845/71‑1 (MQ=255) acagcaGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACAtt < 1:1652865/71‑1 (MQ=255) acagcaGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACAtt < 1:2034712/71‑1 (MQ=255) acagcaGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACAt > 1:3275896/1‑70 (MQ=255) acagcaGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACAt > 1:1441808/1‑70 (MQ=255) acagcaGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACAt > 1:292340/1‑70 (MQ=255) acagcaGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACAt > 1:2696032/1‑70 (MQ=255) acagcaGAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACAt > 1:2053976/1‑70 (MQ=255) cagcagAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGa > 1:154699/1‑45 (MQ=38) cagcagAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGa > 1:3422896/1‑45 (MQ=38) cagcagAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGa > 1:1952810/1‑45 (MQ=38) cagcagAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGa > 1:2237801/1‑45 (MQ=38) cagcagAGAATGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACAtt < 1:1177836/70‑1 (MQ=255) tGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCAc > 1:3334554/1‑44 (MQ=37) tGAAAGGGGAAAAACTGGTATCGCGGCACCGCAGATGCGGTCAc > 1:2610785/1‑44 (MQ=37) aaaGGGG‑AAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACATTATCCGCCGTtata > 1:1937489/1‑70 (MQ=255) aaaGGGG‑AAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACATTATCCGCCGTtata > 1:1356533/1‑70 (MQ=255) aaaGGGG‑AAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACATTATCCGCCGTtata > 1:953084/1‑70 (MQ=255) gg‑AAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACATTATCCGCCGTTATAAAGCg > 1:2314431/1‑70 (MQ=255) gg‑AAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACATTATCCGCCGTTATAAAGCg > 1:1368193/1‑70 (MQ=255) | GTCATTCTTCAATGAAGAAATGAACGAGTTTGTCGATCTGCTGCCAGCACAGCAGAGAATGAAAGGGG‑AAAACTGGTATCGCGGCACCGCAGATGCGGTCACCCAAAACCTCGACATTATCCGCCGTTATAAAGCG > W3110S.gb/4071352‑4071487 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |