| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,155,146:1 | +A | 60.6% | coding (783/870 nt) | prkB ← | predicted phosphoribulokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,155,146 | 1 | . | A | 60.6% | 45.5 / 47.4 | 33 | coding (783/870 nt) | prkB | predicted phosphoribulokinase |
| Reads supporting (aligned to +/- strand): ref base . (2/11); new base A (15/5); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.27e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01 | |||||||||||
CTTCCATCAATCGTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAGGG > W3110S.gb/4155076‑4155202 | cttccATCAATCGTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTAcc < 1:448732/71‑1 (MQ=255) cAATCGTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAAc > 1:715258/1‑71 (MQ=255) aaTCGTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACg > 1:1185112/1‑71 (MQ=255) aaTCGTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACg > 1:660099/1‑71 (MQ=255) aaTCGTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACg > 1:1445463/1‑71 (MQ=255) aaTCGTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACg > 1:416366/1‑71 (MQ=255) gcagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAaatg < 1:1458070/71‑1 (MQ=255) gcagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAaatg < 1:3597383/71‑1 (MQ=255) gcagcaTAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAaatg < 1:3247065/71‑1 (MQ=255) attaattCCATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGc < 1:860926/71‑1 (MQ=255) cATTGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCaa < 1:2780283/70‑1 (MQ=255) aTTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACg < 1:2225539/37‑1 (MQ=255) aTTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACg < 1:2530494/37‑1 (MQ=255) aTTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACg < 1:2776301/37‑1 (MQ=255) aTTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACg < 1:3665501/37‑1 (MQ=255) aTTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACg < 1:1743395/37‑1 (MQ=255) ttGCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTg < 1:723203/42‑1 (MQ=38) ttGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATc < 1:2599589/71‑1 (MQ=255) ttGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATc < 1:1205200/71‑1 (MQ=255) gCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCg > 1:3070956/1‑71 (MQ=255) gCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCg > 1:1105664/1‑71 (MQ=255) gCCAGACCCATTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCg > 1:2799682/1‑71 (MQ=255) cccATTTTGCCGCCCGGTACC‑TCTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATc < 1:294007/63‑1 (MQ=255) aTTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGcc > 1:1216364/1‑51 (MQ=255) aTTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGcc > 1:11573/1‑51 (MQ=255) aTTTTGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGcc > 1:2139322/1‑51 (MQ=255) aTTTTGCCGCCCCGTACCAACTAACGTAATGATGTGGGAAATGAATGAGcc > 1:2968821/1‑51 (MQ=255) ttttGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCa < 1:3598475/71‑1 (MQ=255) ttttGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCa < 1:3605418/71‑1 (MQ=255) ttGCCGCCCGGTGCC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAggg > 1:1916094/1‑71 (MQ=255) ttGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAgg > 1:2955862/1‑71 (MQ=255) ttGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAgg > 1:1675318/1‑71 (MQ=255) ttGCCGCCCGGTACCAACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAgg > 1:1013627/1‑71 (MQ=255) ttGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAggg > 1:1578459/1‑71 (MQ=255) | CTTCCATCAATCGTTGCACCAGCGGCAGCATAATTAATTCCATTGCCAGACCCATTTTGCCGCCCGGTACC‑ACTAACGTATTGATGTGGGAAATGAATGAGCCTTGCAACATCGCCAGCAGCCAGGG > W3110S.gb/4155076‑4155202 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |