| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,428,262:1 | +G | 100% | coding (735/855 nt) | ulaE → | L‑xylulose 5‑phosphate 3‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,428,262 | 1 | . | G | 75.0% | 72.0 / 16.9 | 28 | coding (735/855 nt) | ulaE | L‑xylulose 5‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base . (0/7); new base G (8/13); total (8/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.49e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
ACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAA > W3110S.gb/4428192‑4428324 | aCGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGc > 1:1351798/1‑71 (MQ=255)aCGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGc > 1:1936025/1‑71 (MQ=255)aCGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGc > 1:3563108/1‑71 (MQ=255) gCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCt < 1:1031894/71‑1 (MQ=255) gCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCt < 1:1682040/71‑1 (MQ=255) gCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCt < 1:3196052/71‑1 (MQ=255) gCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCt < 1:310418/71‑1 (MQ=255) gtgtAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTga < 1:634006/71‑1 (MQ=255) gtgtAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTga < 1:531648/71‑1 (MQ=255) gtgtAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTga < 1:3614086/70‑1 (MQ=255) gtgtAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTga < 1:179923/70‑1 (MQ=255) gtgtAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTga < 1:3444055/70‑1 (MQ=255) gtgtAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTga < 1:307615/70‑1 (MQ=255) gtgtAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTga < 1:2970089/70‑1 (MQ=255) tgtAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTga < 1:1442958/70‑1 (MQ=255) gtAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGa > 1:1342419/1‑65 (MQ=255) tAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTgaga < 1:3689957/70‑1 (MQ=255) gTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgt > 1:761169/1‑71 (MQ=255) gATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgtg > 1:3508565/1‑69 (MQ=255) gATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgtg > 1:1585388/1‑69 (MQ=255) gATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGAtgtg > 1:817559/1‑69 (MQ=255) ttCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGc > 1:876158/1‑70 (MQ=255) gAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGaa < 1:2609815/70‑1 (MQ=255) gAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGaa < 1:2066875/70‑1 (MQ=255) gAACCTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGaa < 1:30278/70‑1 (MQ=255) tttCGAAACGCTCAAACAGAGTGGCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGaa < 1:86432/71‑1 (MQ=255) aaaCAGAGTGGCTATTGC‑GGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGTCAGa < 1:1736798/71‑1 (MQ=255) aaaCAGAGTGGCTATTGC‑GGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGa < 1:2488416/71‑1 (MQ=255) gCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGTAAGAcc > 1:2168856/1‑52 (MQ=37) gCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGAcc > 1:1523099/1‑52 (MQ=38) gCTATTGCGGGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGaa < 1:2246567/71‑1 (MQ=255) | ACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC‑GGGCCGTACCTGATTGAGATGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAA > W3110S.gb/4428192‑4428324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |