| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,495,966:1 | +T | 100% | coding (820/924 nt) | ydcI ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,495,966 | 1 | . | T | 95.0% | 107.1 / 0.6 | 40 | coding (820/924 nt) | ydcI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (16/22); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑TCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACA > W3110S.gb/1495899‑1496033 | aaCTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑tt < 1:963510/70‑2 (MQ=255) gCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑TCCCGGaa < 1:1516575/71‑1 (MQ=255) cAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAAcagg < 1:2984490/71‑1 (MQ=255) cAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAAcagg < 1:951699/71‑1 (MQ=255) cAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAAcagg < 1:929013/71‑1 (MQ=255) aagaGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAAcagg < 1:3545897/68‑1 (MQ=255) aagaGAACGTCGCATCTACGCGGGTCAGTATTCCAATAGGTTCGCCTGCACCATGTTCCCGGAAcagg < 1:2201833/68‑1 (MQ=255) gaACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGcc < 1:2458097/71‑1 (MQ=255) cGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGccac > 1:946976/1‑70 (MQ=255) cGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCAGGAACAGGCAGGGccac > 1:1150786/1‑70 (MQ=255) gCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCg > 1:94218/1‑71 (MQ=255) gCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCg > 1:3000389/1‑71 (MQ=255) tCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGt < 1:119598/69‑1 (MQ=255) tCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGt < 1:3397597/69‑1 (MQ=255) tCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGt < 1:235570/69‑1 (MQ=255) tACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAAcaggca > 1:1163395/1‑54 (MQ=39) aCGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAAcaggc < 1:1563561/52‑1 (MQ=39) cgcgGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCAt < 1:2326181/70‑1 (MQ=255) cgcgGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCAt < 1:1729724/70‑1 (MQ=255) cgcgGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCAt < 1:3023202/70‑1 (MQ=255) cgcgGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCAt < 1:827921/70‑1 (MQ=255) cgcgGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCAt < 1:680941/70‑1 (MQ=255) cgcgGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCAt < 1:3556617/70‑1 (MQ=255) cgcgGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCAt < 1:3432399/70‑1 (MQ=255) tCAGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAg > 1:1798927/1‑71 (MQ=255) aGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgt > 1:1827735/1‑71 (MQ=255) aGTATTCCAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGgt > 1:310330/1‑71 (MQ=255) ccAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCtt > 1:2215227/1‑71 (MQ=255) ccAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCtt > 1:1941544/1‑71 (MQ=255) ccAATCGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCtt > 1:1074415/1‑71 (MQ=255) cGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGccacca > 1:1892458/1‑42 (MQ=38) cGGTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGccacca > 1:1606783/1‑42 (MQ=38) ggTTCGCCTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCttt > 1:2013371/1‑66 (MQ=255) ccTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAg < 1:1850886/71‑1 (MQ=255) ccTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAg < 1:3081200/71‑1 (MQ=255) ccTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAg < 1:785023/71‑1 (MQ=255) ccTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAg < 1:908614/71‑1 (MQ=255) ccTGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAg < 1:127362/71‑1 (MQ=255) tGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAg > 1:3645421/1‑69 (MQ=255) tGCACCATGTTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAg > 1:774793/1‑69 (MQ=255) aTG‑TCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACa > 1:3067555/1‑70 (MQ=255) | AACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATG‑TCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACA > W3110S.gb/1495899‑1496033 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |