| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,035,868:1 | +T | 100% | intergenic (‑247/‑320) | yedR ← / → yedS | predicted inner membrane protein/ECK1962:JW5319:b1964; hypothetical protein, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,035,868 | 1 | . | T | 91.7% | 58.4 / 1.2 | 24 | intergenic (‑247/‑320) | yedR/yedS | predicted inner membrane protein/ECK1962:JW5319:b1964; hypothetical protein, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base T (9/13); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
ATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGA > W3110S.gb/2035807‑2035932 | atgCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑tttttttt < 1:3182216/70‑1 (MQ=255) tgCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑tttttttt < 1:737391/69‑1 (MQ=255) tgCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑tttttttt < 1:3587364/69‑1 (MQ=255) gCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑tttttttttat > 1:1112550/1‑68 (MQ=255) cTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑tttttttttat < 1:2529544/70‑4 (MQ=255) gCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCa < 1:1001608/65‑1 (MQ=255) gCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAAtg < 1:1482530/71‑1 (MQ=255) cTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAAtgtg > 1:2972680/1‑71 (MQ=255) cTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAAtgtg > 1:7290/1‑71 (MQ=255) ttttATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑tttttttt < 1:1188883/52‑1 (MQ=255) ttATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑ttttttttta > 1:60939/1‑50 (MQ=255) tCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt < 1:1292535/70‑1 (MQ=255) tCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt < 1:1211168/70‑1 (MQ=255) cGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt < 1:3090284/69‑1 (MQ=255) ttGTTTATATTTTTTGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTaa > 1:3545167/1‑56 (MQ=255) ttttttGGGCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATca > 1:2974475/1‑61 (MQ=255) ggCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGgcgc < 1:402668/71‑1 (MQ=255) gCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGca < 1:1318812/71‑1 (MQ=255) gCCGGCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGca < 1:3413016/71‑1 (MQ=255) ggCATGATGCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCt < 1:1046316/45‑1 (MQ=38) atgatgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAAt > 1:322308/1‑70 (MQ=255) atgatgCCGGC‑TTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCa < 1:1982535/71‑1 (MQ=255) tgatgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATcaca > 1:2556498/1‑48 (MQ=39) tgatgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCa > 1:575822/1‑71 (MQ=255) atgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCAtt < 1:3243033/71‑1 (MQ=255) atgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCAtt < 1:2322582/71‑1 (MQ=255) atgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCAtt < 1:3595712/71‑1 (MQ=255) atgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCAtt > 1:1542211/1‑71 (MQ=255) tgCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTg < 1:941771/71‑1 (MQ=255) gCCGGCTTTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGa > 1:1617877/1‑71 (MQ=255) gC‑TTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCAtt < 1:1916815/64‑1 (MQ=255) | ATGCTTCCGTTATTAGCCTTTTATCGTCTTGTTTATATTTTTTGGGCCGGCATGATGCCGGC‑TTTTTTTTATGCCTTCATTAATGTGCGCCTGATCACACCAGCCGTTTGGCGCAACAATCATTGA > W3110S.gb/2035807‑2035932 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |