| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,169,960:1 | +T | 100% | intergenic (‑3/+166) | yegZ ← / ← yegR | ECK2080:JW5886:b2083; hypothetical protein fragment/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,169,960 | 1 | . | T | 94.7% | 50.9 / ‑2.6 | 19 | intergenic (‑3/+166) | yegZ/yegR | ECK2080:JW5886:b2083; hypothetical protein fragment/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (11/7); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
CGGTGTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑TTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAA > W3110S.gb/2169892‑2170019 | cGGTGTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑tt < 1:140773/71‑1 (MQ=255) tttCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑ttttttt < 1:3028398/71‑1 (MQ=255) tAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCAt > 1:1774842/1‑70 (MQ=255) aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGATCTCTGCTACACGGGCTTATTTTTTTTATGCATAAg > 1:1748486/1‑71 (MQ=255) tatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCAt < 1:1681462/66‑1 (MQ=255) tatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCAt < 1:2769493/66‑1 (MQ=255) atCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGccc > 1:892890/1‑71 (MQ=255) tCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑ttttttt < 1:530505/57‑1 (MQ=255) tCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑ttttttt < 1:442489/57‑1 (MQ=255) ggCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGt > 1:1080574/1‑67 (MQ=255) ggCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGt > 1:2544499/1‑67 (MQ=255) ggCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTc < 1:3046729/71‑1 (MQ=255) ggCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTc < 1:471145/71‑1 (MQ=255) gCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCa > 1:98573/1‑43 (MQ=37) gCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCtt < 1:3536891/69‑1 (MQ=255) gagaACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGa > 1:414659/1‑69 (MQ=255) gaACTCTGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACca > 1:3258556/1‑70 (MQ=255) ctctGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACAGTCTTCCATTGACCACATc < 1:2607741/71‑1 (MQ=255) tGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGa > 1:3477443/1‑70 (MQ=255) tGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGa > 1:1080523/1‑70 (MQ=255) tGCTACACGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGa > 1:1750695/1‑70 (MQ=255) cacGGGCTTA‑TTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGaa < 1:3497366/69‑1 (MQ=255) cGGGCTTATTTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCAtt < 1:482169/52‑1 (MQ=255) | CGGTGTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTA‑TTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAA > W3110S.gb/2169892‑2170019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |