Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 2176983–2178180 2178181 2–1199 57 [0] [0] 54 [gatA]–[gatA] [gatA],insH,[gatA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 21769820 (0.000)45 (1.290) 17/92 NT 100% coding (71/321 nt) gatA galactitol‑specific enzyme IIA component of PTS
?W3110S.gb 2178182 = 0 (0.000)coding (202/315 nt) gatA galactitol‑specific enzyme IIA component of PTS

GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176912‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGC  >  W3110S.gb/2178182‑2178251
                                                                                                                                             
GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                        >  1:2534686/1‑71
GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                        >  1:2925080/1‑71
GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                        >  1:14757/1‑71
GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                        >  1:93107/1‑71
          TCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGC                                                              <  1:2794633/71‑1
                 GCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                         <  1:540652/69‑1
                 GCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                         <  1:579572/69‑1
                 GCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                         <  1:714606/69‑1
                       AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                  >  1:2689840/1‑70
                         TGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTAT                                               <  1:2958765/71‑1
                          GAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATT                                              >  1:1960209/1‑71
                          GAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATT                                              >  1:2826788/1‑71
                          GAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                                >  1:3688121/1‑69
                          GAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACA                                                        >  1:1964575/1‑61
                            ACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                            <  1:1573509/71‑1
                            ACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                            <  1:1424533/71‑1
                            ACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                            <  1:3278856/71‑1
                                TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                          >  1:2412542/1‑69
                                    TGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCG                                     <  1:329930/70‑1
                                    TGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCG                                     <  1:1613528/70‑1
                                     GTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGT                                    <  1:2342510/70‑1
                                     GTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGT                                    <  1:2882229/70‑1
                                      TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTG                                   <  1:2275968/70‑1
                                      TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTG                                   <  1:3459212/70‑1
                                      TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTG                                   <  1:3536504/70‑1
                                      TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTG                                   <  1:3682442/70‑1
                                      TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTG                                   <  1:399347/70‑1
                                      TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                         <  1:2583355/48‑1
                                           CTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                       <  1:95350/45‑1
                                           CTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                       <  1:1767588/45‑1
                                                 AGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAG                                       >  1:2908080/1‑55
                                                  GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                       >  1:3229912/1‑70
                                                  GATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                         <  1:2264915/36‑1
                                                  GATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                         <  1:1463174/36‑1
                                                  GATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                         <  1:2384039/36‑1
                                                  GATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                         <  1:2388313/36‑1
                                                   ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                  <  1:3238828/42‑1
                                                   ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                  <  1:3395835/42‑1
                                                   ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                  <  1:1662226/42‑1
                                                    TAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                       >  1:3166581/1‑36
                                                    TAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                       >  1:3418275/1‑36
                                                    TAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                       >  1:797503/1‑36
                                                    TAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                       >  1:1282197/1‑36
                                                    TAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                       >  1:1197779/1‑36
                                                       ATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCC                 <  1:740279/71‑1
                                                          GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATT                                              >  1:811642/1‑39
                                                          GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATT                                              >  1:957301/1‑39
                                                          GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATT                                              >  1:3023855/1‑39
                                                          GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATT                                              >  1:1202404/1‑39
                                                                GACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAA        <  1:925828/71‑1
                                                                GACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAA        <  1:942008/71‑1
                                                                GACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAA        >  1:130872/1‑71
                                                                   TTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCA                            <  1:3082084/48‑1
                                                                     AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG   >  1:284557/1‑71
                                                                     AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG   >  1:2403129/1‑71
                                                                     AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG   >  1:1596018/1‑71
                                                                      GCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGC  <  1:2089251/71‑1
                                                                                                                                             
GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176912‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGC  >  W3110S.gb/2178182‑2178251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.