| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 15,286 | C→T | 100% | D373D (GAC→GAT) | dnaJ → | chaperone Hsp40, co‑chaperone with DnaK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 15,286 | 0 | C | T | 82.6% | 49.3 / 5.8 | 23 | D373D (GAC→GAT) | dnaJ | chaperone Hsp40, co‑chaperone with DnaK |
| Reads supporting (aligned to +/- strand): ref base C (4/0); new base T (9/10); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGAT > W3110S.gb/15219‑15355 | cAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTgacgac > 1:1261027/1‑68 (MQ=255)cAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTgacgac > 1:139438/1‑68 (MQ=255)cAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCt > 1:272177/1‑70 (MQ=255)cAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCt > 1:1499077/1‑70 (MQ=255)cAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCt > 1:2252194/1‑70 (MQ=255) cGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGAccc > 1:832039/1‑71 (MQ=255) cGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGAccc > 1:1568400/1‑71 (MQ=255) aGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGa < 1:1726249/63‑1 (MQ=255) agCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCt < 1:1184662/46‑1 (MQ=255) agCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCt < 1:822395/46‑1 (MQ=255) agCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCt < 1:3505091/46‑1 (MQ=255) agCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCt < 1:344157/46‑1 (MQ=255) agCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCt < 1:3092769/46‑1 (MQ=255) agCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCt < 1:3048064/46‑1 (MQ=255) agCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCt < 1:2202629/46‑1 (MQ=255) agCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTgg > 1:2104770/1‑71 (MQ=255) agCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCT‑ACCCGCt < 1:2095734/45‑1 (MQ=38) cttcttTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCTaa > 1:1251809/1‑46 (MQ=255) cttTGATGGTGTGAAGAAGTTTTTTGACGACCTGa > 1:2864016/1‑35 (MQ=255) cttTGATGGTGTGAAGAAGTTTTTTGACGACCTGa > 1:3085021/1‑35 (MQ=255) tGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCTAAc > 1:257748/1‑38 (MQ=255) tGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCTAAc > 1:2081577/1‑38 (MQ=255) gTTTTTTGACGATCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGt > 1:1517157/1‑71 (MQ=255) ttttGACGATCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGc < 1:3296909/70‑1 (MQ=255) ttttGACGATCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCg > 1:1740048/1‑71 (MQ=255) gacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGa < 1:318608/71‑1 (MQ=255) acCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGat < 1:2257816/71‑1 (MQ=255) | CAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGAT > W3110S.gb/15219‑15355 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |