| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,198,825 | G→A | 100% | A398T (GCG→ACG) | metG → | methionyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,198,825 | 0 | G | A | 82.9% | 81.1 / 9.8 | 35 | A398T (GCG→ACG) | metG | methionyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (6/0); new base A (15/14); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.12e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTG > W3110S.gb/2198758‑2198876 | ggAAGATTTCGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCgg > 1:2620731/1‑71 (MQ=255) tCGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTa > 1:942769/1‑69 (MQ=255) tCGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTAt > 1:3517308/1‑70 (MQ=255) cGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATc < 1:3312783/70‑1 (MQ=255) cGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATc < 1:3179602/70‑1 (MQ=255) cGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATc > 1:1837866/1‑70 (MQ=255) cGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATc > 1:760264/1‑70 (MQ=255) tgAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGtttt > 1:3385634/1‑71 (MQ=255) tgAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGtttt > 1:2420327/1‑71 (MQ=255) tgAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGtttt < 1:1626370/71‑1 (MQ=255) tgAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGtttt < 1:276092/71‑1 (MQ=255) aaTGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTg < 1:2350871/70‑1 (MQ=255) ccGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGc > 1:62848/1‑71 (MQ=255) atCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCt > 1:76200/1‑71 (MQ=255) atCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCt > 1:353126/1‑71 (MQ=255) atCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCt > 1:2359648/1‑71 (MQ=255) tCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCt < 1:125252/70‑1 (MQ=255) tCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCt < 1:1386421/70‑1 (MQ=255) tCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTg > 1:820248/1‑71 (MQ=255) gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATcaa > 1:2830077/1‑47 (MQ=255) gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGAc > 1:455684/1‑60 (MQ=255) gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGAc > 1:3420794/1‑60 (MQ=255) gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGAc > 1:299214/1‑60 (MQ=255) gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGAc > 1:3275959/1‑60 (MQ=255) gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTgg > 1:3155565/1‑70 (MQ=255) gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTgg > 1:3563991/1‑70 (MQ=255) gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTgg > 1:408977/1‑70 (MQ=255) aaCAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAg > 1:1781693/1‑71 (MQ=255) cAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCg < 1:2762463/71‑1 (MQ=255) cAAAGTGGTTAACATGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCg < 1:1586329/71‑1 (MQ=255) aaaGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCg < 1:1259072/70‑1 (MQ=255) aaaGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGa < 1:3251857/71‑1 (MQ=255) aaCCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTg < 1:2703005/70‑1 (MQ=255) aaCCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTg < 1:245935/70‑1 (MQ=255) cTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGa < 1:1669871/59‑1 (MQ=255) | GGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTG > W3110S.gb/2198758‑2198876 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |