| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,313,823 | T→C | 76.8% | E349G (GAA→GGA) | yojL ← | predicted thiamine biosynthesis lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,313,823 | 0 | T | C | 76.8% | 82.9 / 22.9 | 43 | E349G (GAA→GGA) | yojL | predicted thiamine biosynthesis lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (6/4); new base C (16/17); total (22/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
TTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTATCGCC > W3110S.gb/2313754‑2313878 | ttAAGGATAGACTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACACTCTTGCGCTTTAAttttttt > 1:3448602/1‑70 (MQ=255) aaGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGc > 1:2234785/1‑71 (MQ=255) aaGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGc > 1:2468088/1‑71 (MQ=255) aGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGAc > 1:2889394/1‑69 (MQ=255) tAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGc > 1:2162630/1‑71 (MQ=255) tAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGc > 1:2331529/1‑71 (MQ=255) tAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGc > 1:266785/1‑71 (MQ=255) cccGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTaa < 1:3182544/70‑1 (MQ=255) cccGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTaa < 1:10478/70‑1 (MQ=255) cccGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTaa < 1:195629/70‑1 (MQ=255) cccGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTa < 1:1561072/69‑1 (MQ=255) ccGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAAc < 1:3298038/71‑1 (MQ=255) ggTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtg < 1:2606158/71‑1 (MQ=255) aCCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTgtggt < 1:990253/71‑1 (MQ=255) aTCACGCAAAAACCAACAATCTTGCTCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTgtggt < 1:1567801/68‑1 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTgtggt < 1:582597/68‑1 (MQ=255) tCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAgg < 1:3725306/49‑1 (MQ=255) tCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAgg < 1:139005/49‑1 (MQ=255) cACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACAt > 1:2700431/1‑71 (MQ=255) cACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACAt > 1:1580197/1‑71 (MQ=255) aCGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCt > 1:3556041/1‑52 (MQ=255) aCGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATc > 1:756667/1‑71 (MQ=255) aCGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATc > 1:956518/1‑71 (MQ=255) aCGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa > 1:853275/1‑67 (MQ=255) aaaaCCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTgtggtg < 1:2718590/61‑1 (MQ=255) aaaaCCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTgtggtg < 1:365739/61‑1 (MQ=255) aaCCAACAATCTTGCTCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGAc > 1:2885956/1‑61 (MQ=255) aaCCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGAc > 1:897608/1‑61 (MQ=255) aaCCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGAc > 1:938497/1‑61 (MQ=255) aaCCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGttt > 1:59831/1‑71 (MQ=255) aaCCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGttt > 1:265804/1‑71 (MQ=255) aaTCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGc > 1:3001553/1‑70 (MQ=255) aaTCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGc > 1:3261877/1‑70 (MQ=255) aaTCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCt < 1:371098/71‑1 (MQ=255) aaTCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCt < 1:66551/71‑1 (MQ=255) aTCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTa < 1:504004/71‑1 (MQ=255) aTCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTa > 1:3092896/1‑71 (MQ=255) tCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTa < 1:2511026/40‑1 (MQ=255) cTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTa < 1:1787572/39‑1 (MQ=255) gcgcTTTAATTTTTTCCGCTGACAAGGAAGCTTTTaaa > 1:3262940/1‑38 (MQ=255) gcgcTTTAATTTTTTCCGCTGACAAGGAAGCTTTTaaa > 1:11664/1‑38 (MQ=255) cgcTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTATCGcc < 1:593434/70‑1 (MQ=255) cgcTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTATCGcc < 1:269172/70‑1 (MQ=255) cgcTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTATCGcc < 1:264869/70‑1 (MQ=255) | TTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTATCGCC > W3110S.gb/2313754‑2313878 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |