| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,315,568 | A→G | 100% | G172G (GGT→GGC) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,315,568 | 0 | A | G | 96.1% | 170.4 / ‑1.7 | 51 | G172G (GGT→GGC) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (17/32); total (19/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGG > W3110S.gb/2315502‑2315628 | cAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCt < 1:477697/71‑1 (MQ=255) aCGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGaa > 1:2550401/1‑71 (MQ=255) aCGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGaa > 1:3173759/1‑71 (MQ=255) aCGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGaa > 1:1301929/1‑71 (MQ=255) gttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGt > 1:2656256/1‑71 (MQ=255) gttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGt > 1:2899758/1‑71 (MQ=255) gttagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGt > 1:1823025/1‑71 (MQ=255) tagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGt < 1:783179/54‑1 (MQ=255) ttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCa < 1:3683162/62‑1 (MQ=255) ttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCa < 1:3159059/62‑1 (MQ=255) ccACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTc < 1:526571/71‑1 (MQ=255) caaGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTgg < 1:1113679/40‑1 (MQ=38) cTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTgg < 1:3194719/42‑1 (MQ=255) aGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGccc > 1:2225821/1‑37 (MQ=255) aGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGccc > 1:702326/1‑37 (MQ=255) aGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGcc > 1:3358336/1‑36 (MQ=255) aGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTa > 1:1256370/1‑42 (MQ=255) aGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTa > 1:2925716/1‑42 (MQ=255) aGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTa > 1:2351787/1‑42 (MQ=255) aGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTAc > 1:1721968/1‑43 (MQ=255) aGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTAc > 1:1802518/1‑43 (MQ=255) gTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGcc > 1:3484160/1‑35 (MQ=255) aaaGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGa > 1:2287293/1‑70 (MQ=255) aaGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAg < 1:3201735/68‑1 (MQ=255) aaGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAg < 1:1101618/68‑1 (MQ=255) aaGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAg < 1:250185/68‑1 (MQ=255) ccTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg > 1:2536973/1‑69 (MQ=255) ccTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg > 1:2697905/1‑69 (MQ=255) ttCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGaa < 1:2274918/38‑1 (MQ=255) ttCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGaa < 1:2173009/38‑1 (MQ=255) ttCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGaa < 1:105748/38‑1 (MQ=255) ttCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGaa < 1:553923/38‑1 (MQ=255) ttCACCAGATGCGTTGCCGTTTTTGCCCTGGTACTGaa < 1:3506831/38‑1 (MQ=38) tCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGaa < 1:2401936/37‑1 (MQ=255) ccAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:983070/71‑1 (MQ=255) ccAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:954718/71‑1 (MQ=255) ccAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:1143267/71‑1 (MQ=255) ccAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:654684/71‑1 (MQ=255) ccAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:128758/71‑1 (MQ=255) ccAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:3697049/71‑1 (MQ=255) ccAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:1626285/71‑1 (MQ=255) ccAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:2955392/71‑1 (MQ=255) ccAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:2603994/71‑1 (MQ=255) ccAGATGCGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:3630608/71‑1 (MQ=255) tGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt < 1:1398619/71‑1 (MQ=255) tGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt < 1:3628032/71‑1 (MQ=255) tGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt < 1:1329529/71‑1 (MQ=255) ggTTGCCGTTTTTGCCCTGGAACTGAACACCAAAGTTCAGCCCGTCAACCAGACCGAAGAAGTCAgtgt > 1:1820621/1‑69 (MQ=38) gCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:1464128/70‑1 (MQ=255) gCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:1497965/70‑1 (MQ=255) ccGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc < 1:2046411/59‑1 (MQ=255) | CAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGG > W3110S.gb/2315502‑2315628 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |