| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,328,254 | C→T | 14.3% | D46D (GAC→GAT) | atoD → | acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,328,254 | 0 | C | T | 14.3% | 208.2 / 22.8 | 77 | D46D (GAC→GAT) | atoD | acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (32/34); new base T (6/5); total (38/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAG > W3110S.gb/2328186‑2328324 | ggCGGATTTATGGGGATTTGCACTCCATCCCGCCTGGTTGTAGCATTACTGGAATCTGGTGTTCGCGACCt < 1:2799324/71‑1 (MQ=255)ggCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCt < 1:925609/71‑1 (MQ=255)ggCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCt < 1:1869305/71‑1 (MQ=255)ggCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCt < 1:3004970/71‑1 (MQ=255)ggCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCt < 1:1560010/71‑1 (MQ=255)ggCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCt < 1:3433367/71‑1 (MQ=255) cGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGa < 1:1127012/71‑1 (MQ=255) cGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGa < 1:2023007/71‑1 (MQ=255) cGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGa < 1:3605170/71‑1 (MQ=255) gATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACa < 1:1739121/71‑1 (MQ=255) aTTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACAt < 1:2465052/71‑1 (MQ=255) aTTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACAt < 1:3650258/71‑1 (MQ=255) tttATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACAt < 1:970875/70‑1 (MQ=255) tATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGa > 1:3536713/1‑71 (MQ=255) tATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGa > 1:156290/1‑71 (MQ=255) tATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGa > 1:149841/1‑71 (MQ=255) tATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGa > 1:1434492/1‑71 (MQ=255) tATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGa > 1:960207/1‑71 (MQ=255) tATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGa > 1:2299405/1‑71 (MQ=255) ggCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATa > 1:534859/1‑63 (MQ=255) cTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCaa < 1:3112262/64‑1 (MQ=255) cTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCaa < 1:2854200/64‑1 (MQ=255) cTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCaa < 1:825555/64‑1 (MQ=255) cTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCaa < 1:2549575/64‑1 (MQ=255) cTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCaa < 1:2417793/64‑1 (MQ=255) ccATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATg > 1:260031/1‑64 (MQ=255) ccATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACcgc > 1:2731920/1‑71 (MQ=255) aaTCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACa > 1:993056/2‑50 (MQ=255) tCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGATCTGa < 1:2241109/46‑1 (MQ=255) tCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGa < 1:2134710/46‑1 (MQ=255) tCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGa < 1:2660046/46‑1 (MQ=255) tCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGa < 1:1235251/46‑1 (MQ=255) ccGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGATCTGACa > 1:1634444/1‑46 (MQ=255) ccGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGATCTGACa > 1:1588714/1‑46 (MQ=255) ccGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGATCTGACa > 1:32698/1‑46 (MQ=255) ccGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGATCTGACa > 1:66267/1‑46 (MQ=255) ccGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGATCTGACa > 1:1975453/1‑46 (MQ=255) ccGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTttg > 1:3062063/1‑71 (MQ=255) acGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGATCTGACa > 1:126465/2‑46 (MQ=255) gCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTttgtt < 1:836581/71‑1 (MQ=255) gCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTttgtt < 1:2557182/71‑1 (MQ=255) ggTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATa > 1:2258550/1‑71 (MQ=255) ggTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATa > 1:2876487/1‑71 (MQ=255) gTTGAAGCATTACTGGAATCTGGTGTTCGCGATCTGACATTGATAGCCAATGATACCGCGttt < 1:2562103/63‑1 (MQ=255) gTTGAAGCATTACTGGAATCTGGTGTTCGCGATCTGACATTGATAGCCAATGATACCGCGttt < 1:1238941/63‑1 (MQ=255) gTTGAAGCATTACTGGAATCTGGTGTTCGCGATCTGACATTGATAGCCAATGATACCGCGttt < 1:2392838/63‑1 (MQ=255) ttGAAGCATTACTGGAATCTGGTGTTCGCGATCTGACATTGATAGCCAATGATACCGCGttt < 1:1234368/62‑1 (MQ=255) tGAAGCATTAGTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATa > 1:41898/1‑53 (MQ=255) tGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATa > 1:3099320/1‑53 (MQ=255) tGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATa > 1:157188/1‑53 (MQ=255) aaGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCgg < 1:2440920/70‑1 (MQ=255) aaGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCgg < 1:544067/70‑1 (MQ=255) aaGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCgg < 1:3501240/70‑1 (MQ=255) aaGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGc < 1:1336501/71‑1 (MQ=255) aaGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGc < 1:1991678/71‑1 (MQ=255) aaGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGc < 1:3306895/71‑1 (MQ=255) aaGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGACAATGATACCGCGTTTGTTGATACCGGc < 1:728255/71‑1 (MQ=255) aCTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGa > 1:2916479/1‑42 (MQ=255) aCTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGa > 1:67641/1‑42 (MQ=255) aCTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGa > 1:3156494/1‑42 (MQ=255) aCTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGa > 1:2561674/1‑42 (MQ=255) aCTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGa > 1:203671/1‑42 (MQ=255) aCTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTc > 1:2862500/1‑71 (MQ=255) aCTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTc > 1:1718201/1‑71 (MQ=255) aCTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTc > 1:1640386/1‑71 (MQ=255) aCTGGAATCTGGTGTTCGCGACCTGACATAGATAGCCAATGa > 1:1049205/1‑42 (MQ=255) tGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAAt < 1:3149179/38‑1 (MQ=255) tGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAAt < 1:3018868/38‑1 (MQ=255) gtTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGa > 1:3712144/1‑44 (MQ=255) gtTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGa > 1:153215/1‑44 (MQ=255) gtTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGa > 1:3129387/1‑44 (MQ=255) gtTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGa > 1:2356656/1‑44 (MQ=255) ttCGCGACCTGACATTGATAGCCAATGATACCGCGTttgtt < 1:2690048/41‑1 (MQ=255) ttCGCGACCTGACATTGATAGCCAATGATACCGCGTttgtt < 1:1126776/41‑1 (MQ=255) ttCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAAt > 1:1621342/1‑71 (MQ=255) ttCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGACAAt > 1:3387529/1‑71 (MQ=255) cgcgACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCg > 1:2288437/1‑64 (MQ=255) ccTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAg < 1:382341/71‑1 (MQ=255) ccTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAg < 1:2992232/71‑1 (MQ=255) | GGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAG > W3110S.gb/2328186‑2328324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |