| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,350,543 | G→A | 100% | V337I (GTA→ATA) | nrdA → | ribonucleoside diphosphate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,350,543 | 0 | G | A | 94.5% | 155.5 / 1.1 | 54 | V337I (GTA→ATA) | nrdA | ribonucleoside diphosphate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (22/29); total (24/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTT > W3110S.gb/2350480‑2350601 | ctgctgGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAAt > 1:2606086/1‑71 (MQ=255) ctgGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATcaa > 1:714757/1‑71 (MQ=255) ctgGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATcaa > 1:1799119/1‑71 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATcaaca < 1:1228456/70‑1 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATcaaca < 1:2776189/70‑1 (MQ=255) gtgtTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATcaaca < 1:1563385/70‑1 (MQ=255) ttGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACt < 1:3124911/71‑1 (MQ=255) ttGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACt < 1:2010727/71‑1 (MQ=255) tGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACt < 1:930778/70‑1 (MQ=255) gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg < 1:257834/70‑1 (MQ=255) gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg < 1:2246380/70‑1 (MQ=255) gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg < 1:867758/70‑1 (MQ=255) gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGa > 1:1206173/1‑71 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGTGGTACAAATCAACAAACTGATg < 1:3140658/71‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCGACAAACTGATg < 1:137297/71‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:471140/71‑1 (MQ=255) aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg < 1:669573/71‑1 (MQ=255) gAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGtata > 1:545695/1‑58 (MQ=255) gAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGtata > 1:2690049/1‑58 (MQ=255) gAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGtata > 1:2561669/1‑58 (MQ=255) gAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGaa < 1:435589/71‑1 (MQ=255) ggCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATAcc > 1:1472337/1‑57 (MQ=255) ggCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATAcc > 1:522788/1‑57 (MQ=255) ggCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATAcc > 1:557000/1‑57 (MQ=255) ggCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATAcc > 1:1578953/1‑57 (MQ=255) ggCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATAcc > 1:885447/1‑57 (MQ=255) aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGta < 1:1024917/49‑1 (MQ=255) aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGta < 1:279153/49‑1 (MQ=255) aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGta < 1:3027423/49‑1 (MQ=255) aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGta < 1:401397/49‑1 (MQ=255) aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa < 1:2529875/71‑1 (MQ=255) cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGta > 1:2697798/1‑47 (MQ=255) cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa < 1:3142270/71‑1 (MQ=255) gcgtgcgtCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAg > 1:2354011/1‑69 (MQ=255) gcgtgcgtCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:2349893/1‑70 (MQ=255) tgcgtCATATGGACTACGGGATACAAATCAACAAACTGATGta < 1:178167/43‑1 (MQ=255) tatGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGa < 1:2873001/50‑1 (MQ=255) tatGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGa < 1:2011980/50‑1 (MQ=255) tatGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGa < 1:1374530/50‑1 (MQ=255) tatGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGa < 1:2964166/50‑1 (MQ=255) atGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt > 1:33148/1‑71 (MQ=255) atGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGt > 1:1277027/1‑70 (MQ=255) tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGat < 1:1476081/59‑1 (MQ=255) tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGat < 1:2802578/59‑1 (MQ=255) tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGat < 1:1524787/59‑1 (MQ=255) tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGat < 1:3466368/59‑1 (MQ=255) tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGat < 1:3461858/59‑1 (MQ=255) tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt > 1:3440977/1‑70 (MQ=255) gACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:3117738/1‑56 (MQ=255) gACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:2793256/1‑56 (MQ=255) gACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:59043/1‑56 (MQ=255) gACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:2561739/1‑56 (MQ=255) gACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:2158345/1‑56 (MQ=255) gACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa > 1:1445202/1‑56 (MQ=255) | CTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTT > W3110S.gb/2350480‑2350601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |