| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,397,191 | A→G | 78.3% | F174S (TTC→TCC) | nuoM ← | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,397,191 | 0 | A | G | 78.3% | 176.7 / 42.8 | 83 | F174S (TTC→TCC) | nuoM | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Reads supporting (aligned to +/- strand): ref base A (10/8); new base G (32/32); total (42/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
ACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGA > W3110S.gb/2397128‑2397257 | aCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTg < 1:1024519/69‑1 (MQ=255) aGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGt < 1:2596110/71‑1 (MQ=255) aGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGt < 1:3584516/71‑1 (MQ=255) aGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGt < 1:2801192/71‑1 (MQ=255) aGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGt < 1:736354/71‑1 (MQ=255) aGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGt < 1:1749111/71‑1 (MQ=255) gcCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGa < 1:2665311/70‑1 (MQ=255) gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGttt < 1:149838/70‑1 (MQ=255) gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGtttt < 1:1563396/71‑1 (MQ=255) gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGtttt < 1:3135271/71‑1 (MQ=255) gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGtttt < 1:3139486/71‑1 (MQ=255) tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTgccgcc < 1:2475273/53‑1 (MQ=255) tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTgccgcc < 1:638089/53‑1 (MQ=255) tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTg < 1:1134612/56‑1 (MQ=255) tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTg < 1:1209442/56‑1 (MQ=255) tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGa < 1:2530752/57‑1 (MQ=255) tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGa < 1:2059411/57‑1 (MQ=255) tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGa < 1:3441341/57‑1 (MQ=255) tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGa < 1:2385676/57‑1 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGt > 1:2521704/1‑70 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGt > 1:3704204/1‑70 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGt > 1:3530542/1‑70 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGAt > 1:889501/1‑56 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATa > 1:2011/1‑57 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATa > 1:3085000/1‑57 (MQ=255) aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATa > 1:2638119/1‑57 (MQ=255) tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTg < 1:759079/50‑1 (MQ=255) tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTg < 1:3330502/50‑1 (MQ=255) tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGtttt < 1:3073693/61‑1 (MQ=255) tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGtttt < 1:635039/61‑1 (MQ=255) tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGtttt < 1:2739595/61‑1 (MQ=255) tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCagag > 1:775484/1‑71 (MQ=255) tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCagag > 1:548650/1‑71 (MQ=255) aCCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATAAGCGTTTTACCGTCAGAgg < 1:2080919/70‑1 (MQ=255) ccAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg < 1:3207882/69‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:776773/71‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:2545578/71‑1 (MQ=255) cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:2533585/71‑1 (MQ=255) ccACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATg > 1:2998163/1‑71 (MQ=255) cACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGc > 1:3195849/1‑71 (MQ=255) cACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGc > 1:2011063/1‑71 (MQ=255) tCGCCTGGGTGTAAATGAATGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:418264/63‑1 (MQ=255) tCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg < 1:1652372/60‑1 (MQ=255) cGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGcccc > 1:2464838/1‑70 (MQ=255) gCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTTCCGTCAGAGGCTTTa > 1:3413825/1‑63 (MQ=255) gCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:2938530/1‑63 (MQ=255) gCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:2276739/1‑63 (MQ=255) gCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:479621/1‑63 (MQ=255) gCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:2086598/1‑63 (MQ=255) gCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:1667176/1‑63 (MQ=255) gCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:3092454/1‑63 (MQ=255) gCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:3130919/1‑63 (MQ=255) gCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:792430/1‑63 (MQ=255) gCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:815030/1‑63 (MQ=255) gCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGATTTa > 1:2633790/1‑63 (MQ=255) ccTGGGTGTAAATGAAGTACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:963818/60‑1 (MQ=255) ccTGGGTGTAAATCAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1899365/60‑1 (MQ=255) tGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:324151/1‑60 (MQ=255) tGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:298648/1‑60 (MQ=255) tGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:2446268/1‑60 (MQ=255) tGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:624675/1‑60 (MQ=255) gggTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTaa > 1:2754027/1‑44 (MQ=255) gggTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAc > 1:1532932/1‑45 (MQ=255) gggTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAc > 1:2550354/1‑45 (MQ=255) ggTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAg < 1:1399481/69‑1 (MQ=255) tgtAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCg < 1:1832081/71‑1 (MQ=255) gtAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCg < 1:123277/70‑1 (MQ=255) gtAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCg < 1:748492/70‑1 (MQ=255) tAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1731996/52‑1 (MQ=255) tAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCcaca < 1:1056388/64‑1 (MQ=255) tAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCcaca < 1:2033115/64‑1 (MQ=255) tAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCcaca < 1:3054596/64‑1 (MQ=255) aaTGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg > 1:29703/1‑47 (MQ=255) aaTGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGt > 1:215429/1‑64 (MQ=255) aaTGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGt > 1:2518170/1‑64 (MQ=255) aaTGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGAt > 1:1785856/1‑69 (MQ=255) aaTGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGAt > 1:1744717/1‑69 (MQ=255) aaTGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGAt > 1:3568778/1‑69 (MQ=255) aaTGAAGGACTTGGTTGCCGACGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGt > 1:3187040/1‑64 (MQ=255) aaTGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa > 1:913600/1‑52 (MQ=255) tGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAg > 1:74034/1‑70 (MQ=255) tGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAg > 1:3668046/1‑70 (MQ=255) tGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAg > 1:1071917/1‑70 (MQ=255) aaggaCTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGa < 1:3158492/70‑1 (MQ=255) | ACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGA > W3110S.gb/2397128‑2397257 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |