Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	2,427,890	T→G	100%	I246S (ATT→AGT)	yfcH →	conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,427,890	0	T	G	96.5%	91.7 / ‑4.7	28	I246S (ATT→AGT)	yfcH	conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (16/11);  total (16/12)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCATTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCTTCAGTACTGGTATTA  >  W3110S.gb/2427844‑2427951                                               |                                                              gAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCAt                                       >  1:2677227/1‑71 (MQ=255) gAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCAt                                       >  1:820693/1‑71 (MQ=255) gAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCAt                                       >  1:3633623/1‑71 (MQ=255) gAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCAt                                       >  1:319373/1‑71 (MQ=255) gAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCAt                                       >  1:3010364/1‑71 (MQ=255) gAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCAt                                       >  1:2935970/1‑71 (MQ=255)  aaCAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGAGCGCCAtt                                      >  1:2573292/1‑71 (MQ=255)  aaCAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCAtt                                      >  1:1896987/1‑71 (MQ=255)  aaCAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCAtt                                      >  1:1636900/1‑71 (MQ=255)  aaCAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCAt                                       >  1:2215369/1‑70 (MQ=255)    cAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCg                                    <  1:2189904/71‑1 (MQ=255)         tGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGAc                                  >  1:2407608/1‑68 (MQ=255)         tGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGAc                                  >  1:2799923/1‑68 (MQ=255)           cccATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt                              <  1:1669365/70‑1 (MQ=255)           cccATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt                              <  1:674329/70‑1 (MQ=255)              aTGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt                              <  1:1179522/67‑1 (MQ=255)                 cgcTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGc                       >  1:2052828/1‑71 (MQ=255)                         cATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt                              <  1:3176676/56‑1 (MQ=255)                         cATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt                              <  1:74459/56‑1 (MQ=255)                           tGCGCTGCATCGCCCGGCCATTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCTTCAg             <  1:2730579/71‑1 (MQ=255)                           tGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTaa                            >  1:1581631/1‑56 (MQ=255)                           tGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTaa                            >  1:1180661/1‑56 (MQ=255)                           tGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCTTCAg             <  1:3624794/71‑1 (MQ=255)                           tGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCTTCAg             <  1:1941992/71‑1 (MQ=255)                             cgcTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCTTCAGTa           >  1:3423194/1‑71 (MQ=255)                                      gCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCTTCAGTACTGGTATTa  <  1:1353021/71‑1 (MQ=255)                                      gCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCTTCAGTACTGGTATTa  <  1:569517/71‑1 (MQ=255)                                       cccGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCTTCAGTACTAGTATTa  <  1:1725872/70‑1 (MQ=255)                                               |                                                              GAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCATTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAATCTTCAGTACTGGTATTA  >  W3110S.gb/2427844‑2427951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑