| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,428,249 | A→G | 14.6% | I246T (ATT→ACT) | yfcI ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,428,249 | 0 | A | G | 14.6% | 120.7 / 13.4 | 48 | I246T (ATT→ACT) | yfcI | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (21/20); new base G (4/3); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGAAAAC > W3110S.gb/2428182‑2428315 | cTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATg < 1:450128/70‑1 (MQ=255) cGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATg > 1:516466/1‑60 (MQ=255) cGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATg > 1:3689518/1‑60 (MQ=255) cGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATg > 1:2461053/1‑60 (MQ=255) cGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGtt > 1:2324429/1‑70 (MQ=255) cGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTATCTtgt > 1:966000/1‑71 (MQ=255) gtgtTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTGATCAGTTTCTCCTTTTCTtgt < 1:2581332/70‑1 (MQ=255) gtgtTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTtgt < 1:1120117/70‑1 (MQ=255) gtgtTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTtgt < 1:398188/70‑1 (MQ=255) gtgtTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTtgt < 1:1822154/70‑1 (MQ=255) ttGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCttt < 1:1441029/60‑1 (MQ=255) tGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg > 1:2801559/1‑71 (MQ=255) tGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg > 1:3228169/1‑71 (MQ=255) tGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTgcg > 1:2644981/1‑71 (MQ=255) gCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTc > 1:1068889/1‑70 (MQ=255) ttGCGCCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCTTTTCTtgt < 1:3048809/56‑1 (MQ=255) ttGCGCCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCTTTTCTtgt < 1:2812174/56‑1 (MQ=255) ttGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCt < 1:3078530/71‑1 (MQ=255) ttGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCt < 1:1839738/71‑1 (MQ=255) ttGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCt < 1:2613593/71‑1 (MQ=255) ttGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCt < 1:2716806/71‑1 (MQ=255) ttGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCt < 1:3617649/71‑1 (MQ=255) ttGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCt < 1:2752325/71‑1 (MQ=255) ttGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCt < 1:3485878/71‑1 (MQ=255) ttGCGCCTTCTTCACGTAATCAGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCt < 1:1407490/71‑1 (MQ=255) gcgcCTTCTTCACGTAATCTGTGAGCAGTGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt > 1:3408021/1‑71 (MQ=255) gcgcCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCt > 1:3386075/1‑69 (MQ=255) gcgcCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTa > 1:1838893/1‑70 (MQ=255) gcgcCTTCTTCACGTAATCTGTCAGCAGTGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt > 1:2691601/1‑71 (MQ=255) gcgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTa > 1:1364649/1‑70 (MQ=255) gcgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt > 1:387757/1‑71 (MQ=255) cgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt < 1:1329888/70‑1 (MQ=255) cgcCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTAt < 1:3418682/70‑1 (MQ=255) ccTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCttt < 1:684251/45‑1 (MQ=255) ttcttcACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCtt < 1:2777001/42‑1 (MQ=255) ttcttcACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCtt < 1:639237/42‑1 (MQ=255) ttcttcACGTAATCTGTCAGCAATGGTCATCAGTCTCTCCtt < 1:3331527/42‑1 (MQ=255) tCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGaa > 1:2579971/1‑70 (MQ=255) aGCAGTGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGAAAAc < 1:393962/71‑1 (MQ=255) aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCt > 1:3252265/1‑46 (MQ=255) aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCAc > 1:1827874/1‑53 (MQ=255) aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGaaaa > 1:1458021/1‑70 (MQ=255) aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGaaaa > 1:2732595/1‑70 (MQ=255) aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGaaaa > 1:2080083/1‑70 (MQ=255) aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGaaaa > 1:998379/1‑70 (MQ=255) aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGAAAAc > 1:755971/1‑71 (MQ=255) aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGAAAAc > 1:1319650/1‑71 (MQ=255) aGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGAAAAc > 1:977562/1‑71 (MQ=255) | CTGAGCAATACGCAGGGCTTCTTCGTGTTTGCCCTGCATTGCGCCTTCTTCACGTAATCTGTCAGCAATGGTCATCAGTTTCTCCTTTTCTTGTGGTGCGCGTTCCGCTATCTCACCAATAAATGCACGAAAAC > W3110S.gb/2428182‑2428315 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |