| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,501,495 | G→A | 100% | S135S (TCG→TCA) | ddg → | palmitoleoyl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,501,495 | 0 | G | A | 87.5% | 79.3 / 5.0 | 32 | S135S (TCG→TCA) | ddg | palmitoleoyl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/3); new base A (9/19); total (10/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAAT > W3110S.gb/2501430‑2501561 | gATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTgg < 1:1703996/70‑1 (MQ=255)gATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTgg < 1:2754895/70‑1 (MQ=255)gATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGa < 1:902784/71‑1 (MQ=255)gATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGa < 1:1719010/71‑1 (MQ=255) aCCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACt < 1:3570427/70‑1 (MQ=255) ttAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGggc > 1:3423192/1‑71 (MQ=255) aaaCGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGggcg < 1:83082/70‑1 (MQ=255) cgcACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACt < 1:1038624/60‑1 (MQ=255) gcACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGt < 1:942785/71‑1 (MQ=255) aaaTGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATg > 1:1000736/1‑71 (MQ=255) gTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAat < 1:3277750/71‑1 (MQ=255) tAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAat < 1:3303343/70‑1 (MQ=255) aTGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAatgat > 1:2628096/1‑71 (MQ=255) aTGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAatgat > 1:749727/1‑71 (MQ=255) aTGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAatgat > 1:1715156/1‑71 (MQ=255) aTGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAatga > 1:1643787/1‑70 (MQ=255) aTGGATGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGGCAACCAatgct > 1:708437/1‑69 (MQ=255) gTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTa < 1:1668088/70‑1 (MQ=255) cGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATgg < 1:2670228/41‑1 (MQ=255) gTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAat < 1:936895/56‑1 (MQ=255) gTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAat < 1:2483506/56‑1 (MQ=255) gTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAat < 1:1721976/56‑1 (MQ=255) tCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGccaacc < 1:2034521/52‑1 (MQ=255) tCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGccaacc < 1:258681/52‑1 (MQ=255) cATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTc > 1:2976872/1‑70 (MQ=255) cATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTc > 1:3704029/1‑70 (MQ=255) ttttATGTCACTGGAACTGGGCGGCCGCGTGATgg < 1:2037899/35‑1 (MQ=255) ttttATGTCACTGGAACTGGGCGGCCGCGTGATgg < 1:1783406/35‑1 (MQ=255) ttttATGTCACTGGAACTGGGCGGCCGCGTGATgg < 1:672379/35‑1 (MQ=255) ttATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCcaca > 1:2980910/1‑70 (MQ=255) tGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCCACataat < 1:379510/71‑1 (MQ=255) tGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCCACataat < 1:2908696/71‑1 (MQ=255) | GATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAAT > W3110S.gb/2501430‑2501561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |