| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,512,230 | A→G | 16.1% | W371R (TGG→CGG) | ypdG ← | predicted enzyme IIC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,512,230 | 0 | A | G | 16.1% | 70.5 / 8.2 | 31 | W371R (TGG→CGG) | ypdG | predicted enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base A (16/10); new base G (2/3); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
CAGTTATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAC > W3110S.gb/2512161‑2512290 | cAGTTATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCa < 1:3157124/70‑1 (MQ=255) gTTATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGAt < 1:3473844/71‑1 (MQ=255) tATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAg > 1:1025745/1‑71 (MQ=255) tATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAg > 1:2875017/1‑71 (MQ=255) tGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACt > 1:1913573/1‑71 (MQ=255) aGCGAGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGaa > 1:2112104/1‑71 (MQ=255) gcgATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATa > 1:1159279/1‑54 (MQ=255) gcgATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAg > 1:1395473/1‑55 (MQ=255) gcgATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAg > 1:1692363/1‑55 (MQ=255) tataGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCggg < 1:3071075/53‑1 (MQ=255) taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCg < 1:2930707/42‑1 (MQ=255) taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCg < 1:3115338/42‑1 (MQ=255) taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGaa > 1:2701845/1‑53 (MQ=255) taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCa > 1:2969096/1‑61 (MQ=255) taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCccag > 1:1312669/1‑71 (MQ=255) taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCccag > 1:1308380/1‑71 (MQ=255) taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCccag > 1:3623178/1‑71 (MQ=255) taGACGCCCAGGTTAGTGACCAG‑GGCCATGCCCAGATAGCGGACTCCgg > 1:1288315/1‑49 (MQ=39) cGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCg < 1:847720/38‑1 (MQ=255) cccAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCggg < 1:3074538/45‑1 (MQ=255) ggTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcg > 1:3209924/1‑71 (MQ=255) ggTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcg > 1:2452084/1‑71 (MQ=255) tAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcggcg > 1:1918425/1‑71 (MQ=255) aGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGTAACCATTGCACTGCGCCCAGCCAGACAgcggcg < 1:3378874/70‑1 (MQ=255) aGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcggcg < 1:1073250/70‑1 (MQ=255) aCCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGtt > 1:306256/1‑69 (MQ=255) aCCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGtt > 1:727461/1‑69 (MQ=255) aGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGcc < 1:138161/71‑1 (MQ=255) gCCATGCCGGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc < 1:618572/70‑1 (MQ=255) gCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc < 1:3457781/70‑1 (MQ=255) gCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc < 1:3045715/70‑1 (MQ=255) tGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcggc < 1:850379/54‑1 (MQ=255) | CAGTTATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAC > W3110S.gb/2512161‑2512290 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |