| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,541,664 | G→A | 87.2% | E129K (GAA→AAA) | crr → | glucose‑specific enzyme IIA component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,541,664 | 0 | G | A | 87.2% | 98.1 / 7.6 | 39 | E129K (GAA→AAA) | crr | glucose‑specific enzyme IIA component of PTS |
| Reads supporting (aligned to +/- strand): ref base G (3/2); new base A (16/18); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
GCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAA > W3110S.gb/2541601‑2541734 | gCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAgaga > 1:2351344/1‑70 (MQ=255)gCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAgaga > 1:1342905/1‑70 (MQ=255) gaagaaGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATATGCCGCTGCTGAAAGAGAAAGc > 1:3604627/1‑71 (MQ=255) gaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCaa < 1:2694718/71‑1 (MQ=255) gaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCaa < 1:2094142/71‑1 (MQ=255) aGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTAc < 1:2689690/70‑1 (MQ=255) tGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAagag < 1:3440239/50‑1 (MQ=255) tGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAagag < 1:2286168/50‑1 (MQ=255) tGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAagag < 1:866257/50‑1 (MQ=255) tGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAagag < 1:133766/50‑1 (MQ=255) aaaGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGaa > 1:2343585/1‑50 (MQ=255) aGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTAcc < 1:1628762/61‑1 (MQ=255) ttGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCt > 1:2554567/1‑61 (MQ=255) ttGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCt > 1:1287529/1‑61 (MQ=255) tGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttg > 1:3126045/1‑71 (MQ=255) tGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttg > 1:2132092/1‑71 (MQ=255) cTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCaa < 1:152382/43‑1 (MQ=255) cTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCaa < 1:1679477/43‑1 (MQ=255) cTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCaa < 1:2548204/43‑1 (MQ=255) tCATTGAATTTGATCTGCCGCTGCTTAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCaa > 1:1126421/1‑70 (MQ=255) tCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCaa > 1:3048245/1‑70 (MQ=255) tCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCAGGTTGTTATCTCCaa > 1:683352/1‑70 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACAt > 1:1936891/1‑71 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGt > 1:1036352/1‑40 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAAc < 1:951488/69‑1 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACAt > 1:1195377/1‑71 (MQ=255) ttGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCaa < 1:1082977/37‑1 (MQ=255) ttGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCaa < 1:2907269/37‑1 (MQ=255) ttGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCaa < 1:1638817/37‑1 (MQ=255) ttGAATTTGATCTGCC‑CTGCTGAAAGAGAAAGCCaa < 1:277819/36‑1 (MQ=21) tGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCaa < 1:2860882/36‑1 (MQ=255) gAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTAt > 1:640776/1‑59 (MQ=255) gAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGa > 1:1860176/1‑71 (MQ=255) gAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGa > 1:1460313/1‑71 (MQ=255) tttGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGa < 1:2798876/71‑1 (MQ=255) tGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGaaa > 1:3193089/1‑71 (MQ=255) tGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGaaa > 1:3010914/1‑71 (MQ=255) ctgAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTg < 1:769015/69‑1 (MQ=255) ctgAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACAAAATCAAAGAACTg < 1:265160/69‑1 (MQ=255) gAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaa < 1:3629418/71‑1 (MQ=255) gAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaa < 1:405355/71‑1 (MQ=255) gAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaa < 1:2246621/71‑1 (MQ=255) | GCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAA > W3110S.gb/2541601‑2541734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |