| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,552,318 | T→C | 63.2% | F67S (TTT→TCT) | murP → | fused predicted enzyme IIBC components of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,552,318 | 0 | T | C | 63.2% | 13.6 / 15.7 | 19 | F67S (TTT→TCT) | murP | fused predicted enzyme IIBC components of PTS |
| Reads supporting (aligned to +/- strand): ref base T (4/3); new base C (3/9); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
AAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAG > W3110S.gb/2552278‑2552385 | aaGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCaa > 1:758279/1‑70 (MQ=255)aaGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCaa > 1:2077446/1‑70 (MQ=255)aaGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCaa > 1:2304790/1‑70 (MQ=255) ggCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGc < 1:3441807/71‑1 (MQ=255) ggCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGc < 1:2596676/71‑1 (MQ=255) ggCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGc < 1:3244711/71‑1 (MQ=255) ggCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGc < 1:3367010/71‑1 (MQ=255) gCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCg < 1:1355731/71‑1 (MQ=255) gCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCg < 1:3511124/71‑1 (MQ=255) ttttGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGc < 1:367117/64‑1 (MQ=255) ttGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATgagc > 1:566864/1‑69 (MQ=255) ttGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATgagcg < 1:852047/70‑1 (MQ=255) ttGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATgagcg < 1:785017/70‑1 (MQ=255) ttGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATgagcg < 1:2218527/70‑1 (MQ=255) ttGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATgagcg < 1:2083819/70‑1 (MQ=255) ttGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATgagcg < 1:1710693/70‑1 (MQ=255) ttGACCAGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATgagc > 1:1524013/1‑69 (MQ=255) aGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAgctgct > 1:2249488/1‑71 (MQ=255) aGCGACCAGGTGCAGGTCGTTTCTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAgctgct > 1:175821/1‑71 (MQ=255) tttctGGGCCTGGTAAAGCGCATCGTGCCGCAAACGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAg < 1:1681776/71‑1 (MQ=255) tttctGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAg < 1:3433717/71‑1 (MQ=255) tttctGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAg < 1:689403/71‑1 (MQ=255) tttctGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAg < 1:75709/71‑1 (MQ=255) | AAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAG > W3110S.gb/2552278‑2552385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |