| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,589,664 | A→T | 80.3% | intergenic (+300/‑239) | acrD → / → yffB | aminoglycoside/multidrug efflux system/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,589,664 | 0 | A | T | 80.3% | 150.4 / 32.1 | 66 | intergenic (+300/‑239) | acrD/yffB | aminoglycoside/multidrug efflux system/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (7/6); new base T (33/20); total (40/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
ACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTAAAATACAGA > W3110S.gb/2589602‑2589731 | aCTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAg > 1:2368000/1‑70 (MQ=255)aCTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAg > 1:3542696/1‑70 (MQ=255)aCTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAg > 1:2325382/1‑70 (MQ=255)aCTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAg > 1:3042459/1‑70 (MQ=255) ccTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCaa < 1:2606134/69‑1 (MQ=255) ccTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCaa < 1:1881317/69‑1 (MQ=255) ccTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACa > 1:3095898/1‑71 (MQ=255) ggACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCgaga < 1:2882100/71‑1 (MQ=255) aaaTCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATAtt < 1:391156/71‑1 (MQ=255) aaaTCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATAtt < 1:2358758/71‑1 (MQ=255) aaaTCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATAtt < 1:1663401/71‑1 (MQ=255) tCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCga < 1:1174713/62‑1 (MQ=255) tCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCga < 1:2050465/62‑1 (MQ=255) cTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGata > 1:2381695/1‑65 (MQ=255) cTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGata > 1:1949956/1‑65 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCgaga < 1:2328212/57‑1 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGata > 1:768279/1‑59 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGata > 1:3019542/1‑59 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGata > 1:2178070/1‑59 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCa < 1:835798/70‑1 (MQ=255) aaTACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCgaga < 1:1669104/55‑1 (MQ=255) aaTACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCt < 1:337880/71‑1 (MQ=255) aaTACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCt < 1:997359/71‑1 (MQ=255) tACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGata > 1:2560158/1‑55 (MQ=255) tACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGata > 1:3098046/1‑55 (MQ=255) tACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGata > 1:2517420/1‑55 (MQ=255) tACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGata > 1:39875/1‑55 (MQ=255) aCTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCaa > 1:97469/1‑42 (MQ=255) aCTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCaa > 1:576877/1‑42 (MQ=255) aCTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCaa > 1:2522356/1‑42 (MQ=255) aCTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCaa > 1:3493176/1‑42 (MQ=255) aCTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCaa > 1:3319414/1‑42 (MQ=255) aCTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCaa > 1:3291527/1‑42 (MQ=255) aCTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCaa > 1:1364111/1‑42 (MQ=255) aCTATGCTCCTGCGGTGATGATTTGTATGTGTTACACAGCaa > 1:3487625/1‑42 (MQ=38) cTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCa > 1:2042559/1‑40 (MQ=255) cTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTaa > 1:109103/1‑69 (MQ=255) cTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTaa > 1:1323908/1‑69 (MQ=255) cTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTaa > 1:2758331/1‑69 (MQ=255) tATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAAc < 1:1240612/41‑1 (MQ=255) tATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAAc < 1:673484/41‑1 (MQ=255) aTGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa > 1:2346594/1‑55 (MQ=255) aTGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa > 1:164213/1‑55 (MQ=255) aTGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa > 1:1594932/1‑55 (MQ=255) aTGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa > 1:3424049/1‑55 (MQ=255) ggTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa > 1:3173303/1‑53 (MQ=255) ggTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa > 1:595270/1‑53 (MQ=255) ggTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa > 1:2331596/1‑53 (MQ=255) ggTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTg < 1:3547235/70‑1 (MQ=255) gTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGAt > 1:226161/1‑71 (MQ=255) ccTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGAtt > 1:1336348/1‑70 (MQ=255) cTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTa < 1:2504301/71‑1 (MQ=255) tgatgaTTTGTATGTGTTACACAGCAACATTTCgaga < 1:3537594/37‑1 (MQ=255) atTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTaa < 1:252956/71‑1 (MQ=255) atTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTaa < 1:1146934/71‑1 (MQ=255) atTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTa > 1:1047405/1‑70 (MQ=255) tGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTaaaa < 1:1359382/70‑1 (MQ=255) tGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTaaaa < 1:597441/70‑1 (MQ=255) tGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTaaaa < 1:2669203/70‑1 (MQ=255) tGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTaaaa < 1:3032543/70‑1 (MQ=255) tGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTaaaa < 1:804593/70‑1 (MQ=255) tGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTaaaa < 1:2001652/70‑1 (MQ=255) gATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTAAAATacaga > 1:2814700/1‑69 (MQ=255) gATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTAAAATacaga > 1:2539442/1‑69 (MQ=255) gATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTAAAATacaga > 1:1293751/1‑69 (MQ=255) gATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTAAAATacaga > 1:955520/1‑69 (MQ=255) | ACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTAAAATACAGA > W3110S.gb/2589602‑2589731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |