| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,594,104 | G→A | 100% | H138Y (CAT→TAT) | ypfI ← | predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,594,104 | 0 | G | A | 95.3% | 191.0 / 1.0 | 64 | H138Y (CAT→TAT) | ypfI | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (16/45); total (17/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCAT > W3110S.gb/2594040‑2594166 | gagaATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGAc < 1:1520683/71‑1 (MQ=255) gaATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACaa < 1:3142222/71‑1 (MQ=255) gaATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACaa < 1:3129916/71‑1 (MQ=255) gaATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACaa < 1:3327686/71‑1 (MQ=255) gaATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACaa < 1:2869041/71‑1 (MQ=255) gaATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACaa < 1:2262519/71‑1 (MQ=255) gaATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACaa < 1:2207869/71‑1 (MQ=255) tGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAAt < 1:3262092/71‑1 (MQ=255) tGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAAt > 1:1870888/1‑71 (MQ=255) gCTGGTTTTGGCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATgc < 1:3347621/71‑1 (MQ=255) gCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATgc < 1:3178730/71‑1 (MQ=255) gCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATgc < 1:767635/71‑1 (MQ=255) gCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATgc < 1:1585679/71‑1 (MQ=255) gCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATgc < 1:2397289/71‑1 (MQ=255) tttGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGt > 1:3052258/1‑70 (MQ=255) ttGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCg > 1:2635380/1‑71 (MQ=255) ttGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCg > 1:119239/1‑71 (MQ=255) ttGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCg > 1:1617171/1‑71 (MQ=255) gccgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGc < 1:733572/70‑1 (MQ=255) gccgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGc < 1:3642526/70‑1 (MQ=255) gccgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGc < 1:3706076/70‑1 (MQ=255) gccgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGGTTGAGATACTGGACAAAATGCGGCGTCGc < 1:397223/70‑1 (MQ=255) ccgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCaa < 1:334341/71‑1 (MQ=255) ccgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCaa < 1:587869/71‑1 (MQ=255) ccgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCaa < 1:1731641/71‑1 (MQ=255) ccgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATG‑GGCGTCGCAAt < 1:3093168/71‑1 (MQ=255) cgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAAt < 1:2770303/71‑1 (MQ=255) cgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAAt < 1:1982493/71‑1 (MQ=255) cgccAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAAt < 1:1756223/71‑1 (MQ=255) cAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAgg < 1:3002098/71‑1 (MQ=255) cAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAgg < 1:3187539/71‑1 (MQ=255) cAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAgg < 1:2617594/71‑1 (MQ=255) cAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAgg < 1:3368553/71‑1 (MQ=255) cAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAgg < 1:2621940/71‑1 (MQ=255) cAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAgg < 1:753864/71‑1 (MQ=255) cAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAgg < 1:1799059/71‑1 (MQ=255) ggATAGCCTCGTTATCCGCCGTAAGTACGCGTTTTAGATACTGGACAAAATGCGGCGTCGCAATa < 1:1952803/65‑1 (MQ=255) ggATAGCCTCGTTATCCGCCGTAAGTACGCGTTTTAGATACTGGACAAAATGCGGCGTCGCAATa < 1:1948475/65‑1 (MQ=255) ggATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATa < 1:2436552/65‑1 (MQ=255) ggATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATa < 1:1191250/65‑1 (MQ=255) gATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAg > 1:716421/1‑71 (MQ=255) aTAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATa < 1:734603/63‑1 (MQ=255) aTAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATa < 1:919149/63‑1 (MQ=255) aTAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATa < 1:1649670/63‑1 (MQ=255) aTAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATa < 1:1849857/63‑1 (MQ=255) aTAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATa < 1:2477031/63‑1 (MQ=255) tAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAggg > 1:467205/1‑71 (MQ=255) tAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAggg > 1:1086144/1‑71 (MQ=255) tAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAggg > 1:2899247/1‑71 (MQ=255) aGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAggg > 1:2189642/1‑70 (MQ=255) gCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGc < 1:3574693/70‑1 (MQ=255) gCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGc < 1:2115345/70‑1 (MQ=255) gCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGc < 1:2627835/70‑1 (MQ=255) tCGTTATCCGCCGTAAGTACGCGTTTGAGATACTGGAc < 1:1943663/38‑1 (MQ=255) cGTTATCCGCCGTCAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGc < 1:558434/66‑1 (MQ=255) tCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTc > 1:1732651/1‑69 (MQ=255) tCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTc > 1:113513/1‑69 (MQ=255) tCCGCCGTAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTc > 1:3056420/1‑69 (MQ=255) tAAGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCcagcgcagc < 1:766939/71‑1 (MQ=255) aGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAg > 1:3553828/1‑43 (MQ=255) aGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAg > 1:2043104/1‑43 (MQ=255) aGTACGCGTTTGAGATACTGGACAAAATGCGGCGTCGCAATAg > 1:647732/1‑43 (MQ=255) cgTTTGATATACTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGc < 1:2621972/46‑1 (MQ=255) tGAGATACTGGACAAAATGCGGCGTCGCAATAGGGTAAGGGCAATCACTCCAGCGCAGCGAGTCGGCAt > 1:3202693/1‑69 (MQ=255) | GAGAATGGCTGGTTTTGCCGCCAGAGGATAGCCTCGTTATCCGCCGTAAGTACGCGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCAT > W3110S.gb/2594040‑2594166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |