Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,599,304 G→A 100% M57I (ATG→ATA)  bcp → thiol peroxidase, thioredoxin‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,599,3040GA84.6% 26.8 / ‑0.2 13M57I (ATG→ATA) bcpthiol peroxidase, thioredoxin‑dependent
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (10/1);  total (11/2)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTTCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAAC  >  W3110S.gb/2599240‑2599356
                                                                |                                                    
aTTTCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATGAg                                                >  1:2494223/1‑71 (MQ=255)
aTTTCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATGAg                                                >  1:42080/1‑71 (MQ=255)
   tCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGtt                                              >  1:1253395/1‑70 (MQ=255)
   tCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGtt                                              >  1:3602310/1‑70 (MQ=255)
          gAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGaaaaa                                        <  1:198448/69‑1 (MQ=255)
                cATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCGGGCg                                >  1:1895895/1‑71 (MQ=255)
                             tGTACCGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTggg                   >  1:2446813/1‑71 (MQ=255)
                                 ccGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCGGGCGTTGa                            >  1:208337/1‑58 (MQ=255)
                                 ccGTACAGGCCTGCGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCGGGCGTTGa                            >  1:3610513/1‑58 (MQ=255)
                                         gCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGa       >  1:1011218/1‑71 (MQ=255)
                                              cGGCTTACGCGATAACATAGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAAc  >  1:101104/1‑71 (MQ=255)
                                                          tAACATAGATGAGTTGAAAAAAGCGGGCGTTGATGTGCt                      <  1:1549125/39‑1 (MQ=255)
                                                            aCATAGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAAc  >  1:174653/1‑57 (MQ=255)
                                                                |                                                    
ATTTCTACCCGAAAGCCATGACCCCCGGCTGTACCGTACAGGCCTGCGGCTTACGCGATAACATGGATGAGTTGAAAAAAGCGGGCGTTGATGTGCTGGGTATCAGCACCGATAAAC  >  W3110S.gb/2599240‑2599356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑