| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,636,526 | C→T | 100% | L261L (CTG→CTA) | yfgL ← | protein assembly complex, lipoprotein component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,636,526 | 0 | C | T | 84.4% | 156.1 / 22.2 | 64 | L261L (CTG→CTA) | yfgL | protein assembly complex, lipoprotein component |
| Reads supporting (aligned to +/- strand): ref base C (10/0); new base T (36/18); total (46/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.04e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTT > W3110S.gb/2636467‑2636590 | cgTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaac < 1:718714/71‑1 (MQ=255)cgTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaac < 1:2802878/71‑1 (MQ=255) aTCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTaac < 1:3002869/71‑1 (MQ=255) aTCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTaa > 1:822973/1‑70 (MQ=255) aTCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTaa > 1:2728309/1‑70 (MQ=255) aTCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTaa > 1:1944742/1‑70 (MQ=255) aTCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTaa > 1:1541569/1‑70 (MQ=255) aTCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTaa > 1:1686610/1‑70 (MQ=255) cTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTaacaa > 1:1158571/1‑71 (MQ=255) cTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTaacaa > 1:2259676/1‑71 (MQ=255) cTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTaacaa > 1:1909598/1‑71 (MQ=255) cTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTaacaa > 1:1822478/1‑71 (MQ=255) cACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaac < 1:1103225/52‑1 (MQ=255) cTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaacaa > 1:3037319/1‑52 (MQ=255) cTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAacgac < 1:3434005/68‑1 (MQ=255) tGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAaac > 1:2474131/1‑49 (MQ=255) tGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACgg > 1:1717135/1‑69 (MQ=255) tGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACgg > 1:710103/1‑69 (MQ=255) tGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACgg > 1:2753939/1‑69 (MQ=255) cgcAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGt > 1:726917/1‑71 (MQ=255) cAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGTGAGTCg < 1:2831276/71‑1 (MQ=255) cAGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCg < 1:239262/71‑1 (MQ=255) aGATCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCg < 1:2507163/70‑1 (MQ=255) aTCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaac < 1:3180064/42‑1 (MQ=255) aTCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaac < 1:3461525/42‑1 (MQ=255) aTCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAaac < 1:201659/42‑1 (MQ=255) tCAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc < 1:2195134/71‑1 (MQ=255) cAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgtg > 1:2209201/1‑68 (MQ=255) cAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc > 1:1517053/1‑70 (MQ=255) cAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc > 1:1211704/1‑70 (MQ=255) cAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc > 1:3608948/1‑70 (MQ=255) cAAGCGCCGTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc > 1:1840305/1‑70 (MQ=255) gTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACgg < 1:3082093/52‑1 (MQ=255) gTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACgg < 1:2464352/52‑1 (MQ=255) gTCAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACgg < 1:2695525/52‑1 (MQ=255) cAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAacga > 1:1087577/1‑47 (MQ=255) cAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACAtag > 1:2080444/1‑65 (MQ=255) cAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCg > 1:2177458/1‑67 (MQ=255) cAGGTTACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGc > 1:3724036/1‑68 (MQ=255) ttACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAAc > 1:609718/1‑59 (MQ=255) ttACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTc > 1:221599/1‑66 (MQ=255) ttACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACg > 1:1397499/1‑71 (MQ=255) ttACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACg > 1:754400/1‑71 (MQ=255) ttACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACg > 1:2969635/1‑71 (MQ=255) ttACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACg > 1:22797/1‑71 (MQ=255) ttACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACg > 1:2492366/1‑71 (MQ=255) ttACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACg > 1:3263109/1‑71 (MQ=255) ttACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACg > 1:3690949/1‑71 (MQ=255) ttACCATTAAAGCCTAGCGCGAAAACAACCCCGTTAACAACGACGGGAGTc > 1:2415843/1‑51 (MQ=255) tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAAc > 1:795438/1‑58 (MQ=255) tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCg > 1:2548640/1‑62 (MQ=255) tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACg > 1:2321666/1‑70 (MQ=255) tACCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACg > 1:2361998/1‑70 (MQ=255) aCCATTATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACg > 1:1526086/1‑69 (MQ=255) ttATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGTGAGTCGTGTc < 1:257951/50‑1 (MQ=255) ttATAGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTc < 1:362829/50‑1 (MQ=255) ataGGCTAGCGCGAAAACAACGCCGTTAACAACGACGTGAGTCGTGTCAACATCGCTCAGACGGTCAAttt > 1:160208/1‑71 (MQ=255) ataGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCgt > 1:3341889/1‑45 (MQ=255) ataGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGa > 1:804044/1‑61 (MQ=255) ataGGCTAGCGCGAAAACAACGACGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAAttt > 1:1777693/1‑71 (MQ=255) ataGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCaa > 1:1140695/1‑50 (MQ=255) ataGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCaa > 1:1277485/1‑50 (MQ=255) ataGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCaa > 1:784454/1‑50 (MQ=255) taGGCTAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAAttt < 1:1875808/70‑1 (MQ=255) | CGTTTCCACATAATCTGACCACTGCGCAGATCAAGCGCCGTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTT > W3110S.gb/2636467‑2636590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |