Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,667,558 T→C 100% intergenic (‑6/‑130) hcaR ← / → hcaE DNA‑binding transcriptional regulator/3‑phenylpropionate dioxygenase, large (alpha) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,667,5580TC91.7% 32.9 / ‑3.5 12intergenic (‑6/‑130)hcaR/hcaEDNA‑binding transcriptional regulator/3‑phenylpropionate dioxygenase, large (alpha) subunit
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (10/1);  total (11/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAAC  >  W3110S.gb/2667505‑2667623
                                                     |                                                                 
cAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGaaa                                                   >  1:2031274/1‑70 (MQ=255)
                        aTAGCGTAAATGCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTaa                          >  1:352707/1‑71 (MQ=255)
                                   gCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTcaca                >  1:24449/1‑70 (MQ=255)
                                   gCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTcaca                >  1:3427545/1‑70 (MQ=255)
                                   gCCGTAGTTCCATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTcaca                >  1:908318/1‑70 (MQ=255)
                                            ccATCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaac      >  1:726026/1‑71 (MQ=255)
                                              aTCACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaacca    <  1:1991238/71‑1 (MQ=255)
                                                cACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaaccaac  >  1:2196124/1‑71 (MQ=255)
                                                cACCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaaccaac  >  1:550982/1‑71 (MQ=255)
                                                 aCCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaaccaac  >  1:658919/1‑70 (MQ=255)
                                                 aCCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaaccaac  >  1:1453998/1‑70 (MQ=255)
                                                 aCCTCCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGcaaccaac  >  1:2121387/1‑70 (MQ=255)
                                                     |                                                                 
CAGTGCCTGCGCCACTGCGACGAAATAGCGTAAATGCCGTAGTTCCATCACCTTCCCCTTGTTATCGAAAAAACGTCTCAGCTGGTAGAAATTAACTATTTCACATATTAGCAACCAAC  >  W3110S.gb/2667505‑2667623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑