| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,669,838 | T→C | 75.0% | V92A (GTT→GCT) | hcaC → | 3‑phenylpropionate dioxygenase, predicted ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,669,838 | 0 | T | C | 75.0% | 32.8 / 8.6 | 20 | V92A (GTT→GCT) | hcaC | 3‑phenylpropionate dioxygenase, predicted ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base T (3/2); new base C (7/8); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTGCCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTT > W3110S.gb/2669769‑2669908 | tttGCCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGtt > 1:421960/1‑71 (MQ=255) gCCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGa < 1:9109/70‑1 (MQ=255) aaaaCGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAggt < 1:137010/69‑1 (MQ=255) aGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCa > 1:309986/1‑71 (MQ=255) cGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATc < 1:1596999/71‑1 (MQ=255) aTGCCTTCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAgg > 1:640888/1‑51 (MQ=39) aTGCCTGCCCGCCACCGATCCGCTCACCGCTTATCCAGTACACGCTGAAgg > 1:2117992/1‑51 (MQ=39) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAgg > 1:1613491/1‑51 (MQ=255) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAgg > 1:2367256/1‑51 (MQ=255) accacTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCg < 1:3133527/69‑1 (MQ=255) ccacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATc > 1:1144891/1‑71 (MQ=255) ccacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATc > 1:267108/1‑71 (MQ=255) ccacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATc > 1:3547826/1‑71 (MQ=255) cAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACg < 1:1892791/71‑1 (MQ=255) aGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACg < 1:995138/70‑1 (MQ=255) aGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACg < 1:610129/70‑1 (MQ=255) aGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACg < 1:2818797/70‑1 (MQ=255) aGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACg < 1:138220/70‑1 (MQ=255) aGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGa < 1:2254248/71‑1 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGt > 1:2642853/1‑69 (MQ=255) cacGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTc > 1:454694/1‑70 (MQ=255) ttGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCAttt > 1:3363270/1‑71 (MQ=255) | TTTGCCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTT > W3110S.gb/2669769‑2669908 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |