| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,688,036 | T→C | 71.8% | I43V (ATT→GTT) | yfhG ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,688,036 | 0 | T | C | 71.8% | 74.0 / 30.2 | 46 | I43V (ATT→GTT) | yfhG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (7/6); new base C (16/17); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGA > W3110S.gb/2687977‑2688103 | cAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTctg < 1:2135852/71‑1 (MQ=255) gATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtttt > 1:2969164/1‑56 (MQ=255) gATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtttt > 1:2597143/1‑56 (MQ=255) gATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtttt > 1:2453538/1‑56 (MQ=255) gATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtttt > 1:1943055/1‑56 (MQ=255) gATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAActtttt > 1:1822019/1‑56 (MQ=255) gATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTAt < 1:3045875/71‑1 (MQ=255) gATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTAt < 1:2357200/71‑1 (MQ=255) gATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTAt < 1:2244010/71‑1 (MQ=255) gATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTAt < 1:2006952/71‑1 (MQ=255) gATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTAt < 1:616276/71‑1 (MQ=255) aTGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTAt > 1:410653/1‑70 (MQ=255) aTGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATc < 1:353243/71‑1 (MQ=255) aTGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATc < 1:2098183/71‑1 (MQ=255) aTGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATc < 1:930363/71‑1 (MQ=255) gTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCaa > 1:2100574/1‑71 (MQ=255) gTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCaa > 1:569077/1‑71 (MQ=255) gTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCaa > 1:3022696/1‑71 (MQ=255) gCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAAtg < 1:3227831/70‑1 (MQ=255) cATTCAGTTGAAAGATAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAAtggctg > 1:2878707/1‑70 (MQ=255) cATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTctg > 1:1104467/1‑52 (MQ=255) cATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAAtggctgg > 1:93326/1‑71 (MQ=255) cATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAAtggctgg > 1:2876018/1‑71 (MQ=255) cATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAAtggctgg > 1:2620401/1‑71 (MQ=255) cATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAAtggctg > 1:1053986/1‑70 (MQ=255) ttCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATggctggct > 1:371018/1‑71 (MQ=255) ttCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATggctggct > 1:3381444/1‑71 (MQ=255) tAGAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATtg > 1:3237909/1‑71 (MQ=255) gAAAGATAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATtgt < 1:501489/70‑1 (MQ=255) gAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttctt > 1:3051270/1‑40 (MQ=255) gAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttctt > 1:3359309/1‑40 (MQ=255) gAAAGATAGTCAGCCAGTTGATAAACCGGAACTTTttctt > 1:2489316/1‑40 (MQ=255) aGATAGTCAGCCAGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGAtt > 1:969008/1‑71 (MQ=255) aGTTGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGa > 1:2630752/1‑57 (MQ=255) tGATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAg > 1:1131576/1‑71 (MQ=255) gATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAg > 1:2974332/1‑70 (MQ=255) gATAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAg > 1:548565/1‑70 (MQ=255) aTAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGc < 1:1810123/70‑1 (MQ=255) aTAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGc < 1:1154260/70‑1 (MQ=255) aTAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGc < 1:1726717/70‑1 (MQ=255) aTAAACCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGc < 1:2850532/70‑1 (MQ=255) aaaCCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCaa > 1:2536137/1‑70 (MQ=255) aaCCGGAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATg > 1:1989967/1‑71 (MQ=255) aCCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGc < 1:1884875/71‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:1077421/71‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:860998/71‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:1359535/71‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:1722547/71‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:434903/71‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:3542677/71‑1 (MQ=255) gAACTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGa < 1:2262270/71‑1 (MQ=255) | CAGCGCCCAGATGTCGCTACATTCAGTAGAAAGATAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGA > W3110S.gb/2687977‑2688103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |