| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,697,845 | G→A | 100% | G144D (GGT→GAT) | yfhH → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,697,845 | 0 | G | A | 97.2% | 205.9 / ‑1.7 | 70 | G144D (GGT→GAT) | yfhH | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (37/31); total (37/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGA > W3110S.gb/2697777‑2697911 | aCTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATc > 1:3209024/1‑71 (MQ=255)aCTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATc > 1:3587557/1‑71 (MQ=255)aCTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATc > 1:3181540/1‑71 (MQ=255)aCTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATc > 1:1781156/1‑71 (MQ=255)aCTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATc > 1:559662/1‑71 (MQ=255)aCTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATc > 1:453863/1‑71 (MQ=255)aCTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATc > 1:457914/1‑71 (MQ=255) cATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGCCCGGTATTGGCGCTTCGGATCtggt > 1:3053662/1‑71 (MQ=255) cATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCtggt > 1:2491826/1‑71 (MQ=255) cATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCtggt > 1:3310093/1‑71 (MQ=255) cATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCtggt > 1:712911/1‑71 (MQ=255) cATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCtggt > 1:3180518/1‑71 (MQ=255) aTGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCtggtg < 1:609810/71‑1 (MQ=255) aTGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCtggtg < 1:364398/71‑1 (MQ=255) aTGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCtggtg < 1:802985/71‑1 (MQ=255) aTGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTCGCGCTTCGGATCtggtg < 1:415921/71‑1 (MQ=255) aaTGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATc > 1:1423540/1‑63 (MQ=255) aaTGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATc > 1:1217486/1‑63 (MQ=255) aaCAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACt > 1:1497889/1‑71 (MQ=255) aaCAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACt > 1:3536733/1‑71 (MQ=255) aCAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACtt > 1:329141/1‑71 (MQ=255) cAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACttt < 1:943334/71‑1 (MQ=255) cAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACttt < 1:2408219/71‑1 (MQ=255) aTGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCaaaa > 1:2595706/1‑65 (MQ=255) aTGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGc > 1:94154/1‑71 (MQ=255) aTGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACATTGc > 1:391758/1‑71 (MQ=255) ttGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTg > 1:3303638/1‑71 (MQ=255) ttGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTg > 1:3314234/1‑71 (MQ=255) ttGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTg > 1:1032334/1‑71 (MQ=255) ttGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTg > 1:3143453/1‑71 (MQ=255) ttGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTg > 1:1124359/1‑71 (MQ=255) gcTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAAc < 1:400702/59‑1 (MQ=255) tGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtga > 1:970584/1‑71 (MQ=255) tGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtga > 1:3693924/1‑71 (MQ=255) tGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtga > 1:3606532/1‑71 (MQ=255) tGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtga > 1:3444514/1‑71 (MQ=255) tGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtga > 1:3439247/1‑71 (MQ=255) tGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCt < 1:923314/69‑1 (MQ=255) ggATAATTCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGAtt < 1:2182182/71‑1 (MQ=255) tCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTc > 1:271711/1‑70 (MQ=255) tCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTc > 1:2154282/1‑70 (MQ=255) tCTGACCGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTc > 1:3706494/1‑70 (MQ=255) tCTGACAGGTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTc > 1:679354/1‑70 (MQ=255) aCCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAAt < 1:1909122/69‑1 (MQ=255) gTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCtt < 1:3401144/61‑1 (MQ=255) gTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCtt < 1:774883/61‑1 (MQ=255) gTATTGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCtt < 1:1697501/61‑1 (MQ=255) ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:2035766/70‑1 (MQ=255) ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:3242927/70‑1 (MQ=255) ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:431231/70‑1 (MQ=255) ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGc < 1:378453/70‑1 (MQ=255) ttGGCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCa < 1:3179689/71‑1 (MQ=255) ggCGCTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAAtgctgc > 1:2972237/1‑65 (MQ=255) cTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTgcgc < 1:1980107/71‑1 (MQ=255) cTTCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTgcgc < 1:1343521/71‑1 (MQ=255) tCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGa > 1:2202709/1‑43 (MQ=255) tCGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGa > 1:1019339/1‑43 (MQ=255) cGGATCTGTTGGCGCAAAACTTTGCCTGGAAGCtgatg < 1:3366856/38‑1 (MQ=37) cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtgatg < 1:3042275/38‑1 (MQ=255) cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtgatg < 1:1112489/38‑1 (MQ=255) cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCtgatg < 1:446061/38‑1 (MQ=255) cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGa < 1:492168/70‑1 (MQ=255) cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGa < 1:1543204/70‑1 (MQ=255) cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGa < 1:2281896/70‑1 (MQ=255) cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGa < 1:1119815/70‑1 (MQ=255) cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGa < 1:3420267/70‑1 (MQ=255) cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGa < 1:367013/70‑1 (MQ=255) cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGa < 1:1049383/70‑1 (MQ=255) cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGa < 1:1822772/70‑1 (MQ=255) cGGATCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGa < 1:3189103/70‑1 (MQ=255) | ACTGCATGAATGCGTAACAATGTTGCGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGA > W3110S.gb/2697777‑2697911 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |