Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,717,229 G→A 100% intergenic (‑45/‑162) yfiF ← / → trxC predicted methyltransferase/thioredoxin 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,717,2290GA89.3% 70.3 / 2.0 28intergenic (‑45/‑162)yfiF/trxCpredicted methyltransferase/thioredoxin 2
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (17/8);  total (19/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAG  >  W3110S.gb/2717174‑2717293
                                                       |                                                                
tcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGc                                                    <  1:425904/70‑1 (MQ=255)
tcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGc                                                    >  1:329500/1‑70 (MQ=255)
  atCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCAt                                                 <  1:1463730/71‑1 (MQ=255)
  atCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCAt                                                 <  1:3617918/71‑1 (MQ=255)
        cATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTc                                           >  1:1961902/1‑71 (MQ=255)
        cATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACACGAAGTCCGCGCGGTTCGCCATGATTTc                                           >  1:2772447/1‑71 (MQ=255)
         aTGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCg                                          <  1:2187187/71‑1 (MQ=255)
                 ctcCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCaaa                                   <  1:797410/70‑1 (MQ=255)
                  tcCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGc                                 >  1:759131/1‑71 (MQ=255)
                  tcCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGc                                 >  1:829825/1‑71 (MQ=255)
                  tcCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGc                                 >  1:676397/1‑71 (MQ=255)
                    caccaGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCt                               >  1:186876/1‑71 (MQ=255)
                        aGCGCTGCGCGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGa                           >  1:236306/1‑71 (MQ=255)
                              gcgcGAAGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCa                     >  1:2650743/1‑71 (MQ=255)
                                    aGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTa               <  1:2294415/71‑1 (MQ=255)
                                    aGCGCGCAGATTACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTa               <  1:1663846/71‑1 (MQ=255)
                                            gATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATa        >  1:873401/1‑70 (MQ=255)
                                              ttACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCAt                    >  1:1001000/1‑56 (MQ=255)
                                               tACCCGAAATCCTCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaaaa     >  1:3399849/1‑70 (MQ=255)
                                               tACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGa                           >  1:356936/1‑48 (MQ=255)
                                               tACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTa               >  1:526236/1‑60 (MQ=255)
                                               tACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaaaa     >  1:301278/1‑70 (MQ=255)
                                               tACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaaaa     >  1:549712/1‑70 (MQ=255)
                                               tACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaaaa     >  1:2688208/1‑70 (MQ=255)
                                               tACCCGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaaaa     >  1:1495112/1‑70 (MQ=255)
                                               tACCCGAAATCCACGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGa                           >  1:3520137/1‑48 (MQ=255)
                                                 cccGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAg  <  1:1287615/71‑1 (MQ=255)
                                                 cccGAAATCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAg  <  1:1062996/71‑1 (MQ=255)
                                                       |                                                                
TCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAG  >  W3110S.gb/2717174‑2717293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑