Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 2,773,435 | T→C | 78.7% | L488L (TTG→CTG) | yfjW → | predicted inner membrane protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 2,773,435 | 0 | T | C | 78.7% | 130.8 / 30.1 | 61 | L488L (TTG→CTG) | yfjW | predicted inner membrane protein |
Reads supporting (aligned to +/- strand): ref base T (8/5); new base C (14/34); total (22/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.96e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGTTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTT > W3110S.gb/2773367‑2773495 | aGTTTAGTATCAACGGGAAACATTTGTCGTTTAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:615546/71‑1 (MQ=255) ttAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTAt > 1:1125743/1‑71 (MQ=255) tAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg > 1:606776/1‑71 (MQ=255) tAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg > 1:293239/1‑71 (MQ=255) tAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg > 1:1909712/1‑71 (MQ=255) tAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg > 1:1868395/1‑71 (MQ=255) tAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg > 1:611040/1‑71 (MQ=255) tATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:2051597/64‑1 (MQ=255) tATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:3160871/64‑1 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGAc > 1:2188748/1‑69 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGAc > 1:3331964/1‑69 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGAc > 1:19011/1‑69 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa < 1:2229893/64‑1 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa < 1:394295/64‑1 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGAc > 1:3236374/1‑69 (MQ=255) tCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGAc > 1:3667302/1‑69 (MQ=255) aaCGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa > 1:406893/1‑62 (MQ=255) aaCGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa > 1:1745154/1‑62 (MQ=255) aaCGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa > 1:2203253/1‑62 (MQ=255) aaCGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGt > 1:1970166/1‑71 (MQ=255) aaCGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGt > 1:3323960/1‑71 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:1189575/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:812267/54‑1 (MQ=255) aaaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:878017/54‑1 (MQ=255) cATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTtaataa > 1:2629256/1‑68 (MQ=255) tttGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa < 1:1343398/51‑1 (MQ=255) tCGTTAAGGCCAGAATCGGAGATTATTATTTGTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCaaaaa < 1:343909/71‑1 (MQ=255) tCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATg < 1:28245/49‑1 (MQ=255) tCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCaaaaa < 1:1042096/71‑1 (MQ=255) ttAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACt < 1:672172/50‑1 (MQ=255) ttAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACt < 1:2973465/50‑1 (MQ=255) ttAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACt < 1:2481403/50‑1 (MQ=255) ttAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACt < 1:2096241/50‑1 (MQ=255) ttAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:834569/42‑1 (MQ=255) ttAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTg < 1:2383201/42‑1 (MQ=255) cAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTATTTAATAAAAGCAAAAAAATCAAAata < 1:3523341/71‑1 (MQ=255) cAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAata < 1:3296752/71‑1 (MQ=255) aGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatat < 1:3681767/71‑1 (MQ=255) gAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatata < 1:2613848/71‑1 (MQ=255) gAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatata < 1:3301897/71‑1 (MQ=255) tCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATgg < 1:1227120/71‑1 (MQ=255) gagaTTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGcag < 1:2958376/71‑2 (MQ=255) gagaTTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGcac < 1:3229120/71‑1 (MQ=255) gagaTTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGcac < 1:1479731/71‑1 (MQ=255) gagaTTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGcac < 1:457680/71‑1 (MQ=255) gaTTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCAcag > 1:3455607/1‑71 (MQ=255) gaTTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCAcag > 1:2739313/1‑71 (MQ=255) gaTTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCAcag > 1:1685974/1‑71 (MQ=255) attattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAat < 1:1230521/59‑1 (MQ=255) attattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGcac < 1:673120/68‑1 (MQ=255) attattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGcac < 1:1858155/68‑1 (MQ=255) attattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGcac < 1:2540197/68‑1 (MQ=255) ttattattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGcac < 1:996482/67‑1 (MQ=255) attattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCAcag > 1:398605/1‑67 (MQ=255) attattTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCAcag > 1:2057367/1‑67 (MQ=255) aaGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGttt < 1:1954078/69‑1 (MQ=255) aGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaa < 1:39295/42‑1 (MQ=255) aGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaa < 1:3705004/42‑1 (MQ=255) aGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaa < 1:2242720/42‑1 (MQ=255) aGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaa < 1:1253942/42‑1 (MQ=255) aGGGAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCaa < 1:112399/42‑1 (MQ=255) | AGTTTAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTT > W3110S.gb/2773367‑2773495 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |