| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,794,102 | G→A | 100% | L398L (TTG→TTA) | gabP → | gamma‑aminobutyrate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,794,102 | 0 | G | A | 96.0% | 139.3 / ‑1.4 | 50 | L398L (TTG→TTA) | gabP | gamma‑aminobutyrate transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (23/25); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGT > W3110S.gb/2794035‑2794167 | taGTCATCGCCGTTTGACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg < 1:551243/70‑1 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg < 1:2822366/70‑1 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg < 1:2814545/70‑1 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACg < 1:569687/70‑1 (MQ=255) gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCt > 1:2238415/1‑71 (MQ=255) aGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTgg < 1:2116118/71‑1 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCg < 1:3200145/66‑1 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCg < 1:2549916/66‑1 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCg < 1:1441074/66‑1 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCg < 1:3635399/66‑1 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCt < 1:1785846/71‑1 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCt < 1:865950/71‑1 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCt < 1:960699/71‑1 (MQ=255) ttGCGGATACGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCt < 1:3315508/71‑1 (MQ=255) gCGGATGAGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTc < 1:3560204/70‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTc < 1:611663/69‑1 (MQ=255) aTGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTg < 1:3672140/71‑1 (MQ=255) aTGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTg < 1:1153590/71‑1 (MQ=255) cgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCg > 1:1074272/1‑57 (MQ=255) cgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCg > 1:3358391/1‑57 (MQ=255) cgcAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCg > 1:221241/1‑57 (MQ=255) aaaaTTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGt > 1:898674/1‑71 (MQ=255) aaaaTTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGt > 1:988846/1‑71 (MQ=255) aaaaTTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGt > 1:3035266/1‑71 (MQ=255) aaaaTTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGt > 1:2920038/1‑71 (MQ=255) aaaaTTCTGCGAGCAGAAGGAAGCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACATGGCTAg > 1:2528331/1‑70 (MQ=255) gaaggaagCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCt < 1:1876482/44‑1 (MQ=255) aggaagCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCt > 1:304395/1‑71 (MQ=255) aggaagCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCt > 1:3333774/1‑71 (MQ=255) ggaagCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt > 1:1061141/1‑71 (MQ=255) ggaagCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt < 1:3533436/71‑1 (MQ=255) ggaagCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt > 1:1909764/1‑71 (MQ=255) ggaagCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt > 1:36869/1‑71 (MQ=255) agCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTtgt > 1:3433912/1‑71 (MQ=255) agCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTtgt < 1:119116/71‑1 (MQ=255) agCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTtgt > 1:764060/1‑71 (MQ=255) agCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTtgt > 1:1806580/1‑71 (MQ=255) gCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCt < 1:3310673/37‑1 (MQ=255) gCGAAATTCGCTTACGAATGTGGCTTTATCCGTGGCt < 1:1118891/37‑1 (MQ=255) gAAATTCGCTTACGAATGTGGCTTTATCCGTGGCt > 1:714137/1‑35 (MQ=255) gAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCa > 1:2511883/1‑37 (MQ=255) gAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCa > 1:1533951/1‑37 (MQ=255) gAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCa > 1:2149420/1‑37 (MQ=255) gAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCa > 1:2501445/1‑37 (MQ=255) aaTTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTgg < 1:1274533/71‑1 (MQ=255) aaTTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTgg < 1:70952/71‑1 (MQ=255) aaTTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTgg < 1:1194814/71‑1 (MQ=255) aaTTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTgg < 1:3174066/71‑1 (MQ=255) cGCTTACGAATGTGGCTTTATCCGTGGCTCGCCTGGCTAGTCATAGGCTTTATTACCTTTGTGTTGGTAg > 1:455281/1‑70 (MQ=255) gCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGt < 1:3048099/70‑1 (MQ=255) | TAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTTTGTGTTGGTAGT > W3110S.gb/2794035‑2794167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |