| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,821,480 | T→C | 76.0% | T316A (ACC→GCC) | recA ← | DNA strand exchange and recombination protein with protease and nuclease activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,821,480 | 0 | T | C | 76.0% | 94.5 / 28.4 | 50 | T316A (ACC→GCC) | recA | DNA strand exchange and recombination protein with protease and nuclease activity |
| Reads supporting (aligned to +/- strand): ref base T (8/4); new base C (12/26); total (20/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGC > W3110S.gb/2821412‑2821548 | gaAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGctt > 1:2985916/1‑71 (MQ=255)gaAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGctt > 1:1724953/1‑71 (MQ=255) gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTATCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCttt > 1:3306567/1‑71 (MQ=255) cGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGc > 1:3525766/1‑70 (MQ=255) cGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGc > 1:158038/1‑70 (MQ=255) cGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGc > 1:2713232/1‑70 (MQ=255) tGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAgg > 1:1448143/1‑69 (MQ=255) gCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGc > 1:254351/1‑64 (MQ=255) gCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGc > 1:1593623/1‑64 (MQ=255) cTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAg > 1:3593279/1‑70 (MQ=255) cTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt < 1:3465806/71‑1 (MQ=255) cTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGt > 1:1292612/1‑71 (MQ=255) agcagcAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCg < 1:1832089/70‑1 (MQ=255) agcagcAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCg < 1:771541/70‑1 (MQ=255) agcagcAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCg < 1:3504196/70‑1 (MQ=255) agcagcAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCg < 1:931165/70‑1 (MQ=255) agcagcAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGc < 1:3212327/71‑1 (MQ=255) gcagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCa < 1:1193140/71‑1 (MQ=255) gcagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCa < 1:944131/71‑1 (MQ=255) gcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGcc < 1:1888979/57‑1 (MQ=255) gcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGcc < 1:89212/57‑1 (MQ=255) caACTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCg < 1:1127398/71‑1 (MQ=255) aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc > 1:2484975/1‑71 (MQ=255) aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc > 1:2604016/1‑71 (MQ=255) aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc > 1:3003191/1‑71 (MQ=255) cTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt < 1:522637/71‑1 (MQ=255) cTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt < 1:2100480/71‑1 (MQ=255) cTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt < 1:583791/71‑1 (MQ=255) cTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt < 1:345526/71‑1 (MQ=255) cACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa > 1:1330370/1‑71 (MQ=255) cACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCAAGGCAGTCGCATTCGCTTTa > 1:2791118/1‑71 (MQ=255) aCGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAc < 1:1271655/71‑1 (MQ=255) aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCttt < 1:237819/69‑1 (MQ=255) aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCttt < 1:184994/69‑1 (MQ=255) aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCttt < 1:2806008/69‑1 (MQ=255) aCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa < 1:3118371/70‑1 (MQ=255) aCGTACTTTCTTCTCGATCTCTTTCGCGGCTCCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCttt < 1:1886630/69‑1 (MQ=255) tACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCt > 1:3332367/1‑71 (MQ=255) tACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCt > 1:1474133/1‑71 (MQ=255) tACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACACt > 1:2307654/1‑71 (MQ=255) cttctCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCg < 1:3097790/70‑1 (MQ=255) cttctCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCg < 1:64015/70‑1 (MQ=255) cttctCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCg < 1:3101926/70‑1 (MQ=255) ctcGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGa < 1:530590/68‑1 (MQ=255) ctcGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGa < 1:465625/68‑1 (MQ=255) ctcGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGa < 1:710813/68‑1 (MQ=255) aTCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCg < 1:99054/63‑1 (MQ=255) tttCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCAc < 1:3465085/68‑1 (MQ=255) tttCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCAc < 1:3362752/68‑1 (MQ=255) ggTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCa > 1:525276/1‑61 (MQ=255) ggTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGc > 1:722429/1‑71 (MQ=255) ggTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGc > 1:2268433/1‑71 (MQ=255) | GAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGC > W3110S.gb/2821412‑2821548 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |